Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index

Beffagna, G; Sammarco, A; Bedin, C; Romualdi, C; Mainenti, M; Mollo, A; Cavicchioli, L; Ferro, S; Trez, D; De Maria, R; Nitti, D; Saccani, A; Campanella, M; Agostini, M; Zappulli, V

Abstract

Circulating cell-free DNA (cfDNA) has been considered an interesting diagnostic/prognostic plasma biomarker in tumor-bearing subjects. In cancer patients, cfDNA can hypothetically derive from tumor necrosis/apoptosis, lysed circulating cells, and some yet unrevealed mechanisms of active release. This study aimed to preliminarily analyze cfDNA in dogs with canine mammary tumors (CMTs). Forty-four neoplastic, 17 non-neoplastic disease-bearing, and 15 healthy dogs were recruited. Necrosis and apoptosis were also assessed as potential source of cfDNA on 78 CMTs diagnosed from the 44 dogs. The cfDNA fragments and integrity index significantly differentiated neoplastic versus non-neoplastic dogs (P

Citation

Beffagna, G., Sammarco, A., Bedin, C., Romualdi, C., Mainenti, M., Mollo, A., Cavicchioli, L., Ferro, S., Trez, D., De Maria, R., Nitti, D., Saccani, A., Campanella, M., Agostini, M., & Zappulli, V. (2017). Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index. PLoS ONE, 12(1), e0169454. https://doi.org/10.1371/journal.pone.0169454

• Journal Article Type: Article
• Acceptance Date: Dec 16, 2016
• Publication Date: Jan 20, 2017
• Deposit Date: Feb 17, 2017
• Publicly Available Date: Feb 17, 2017
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 1
• Pages: e0169454
• DOI: https://doi.org/10.1371/journal.pone.0169454
• Public URL: https://rvc-repository.worktribe.com/output/1393715