Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination

Crawford, A H; Tripathi, R B; Foerster, S; McKenzie, I; Kougioumtzidou, E; Grist, M; Richardson, W D; Franklin, R J

doi:10.1016/j.ajpath.2015.11.005

Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination

Crawford, A H; Tripathi, R B; Foerster, S; McKenzie, I; Kougioumtzidou, E; Grist, M; Richardson, W D; Franklin, R J

Authors

A H Crawford

R B Tripathi

S Foerster

I McKenzie

E Kougioumtzidou

M Grist

W D Richardson

R J Franklin

Abstract

Remyelination is the regenerative response to demyelination. Although the oligodendrocyte progenitor is established as the major source of remyelinating cells, there is no conclusive evidence on whether mature, differentiated oligodendrocytes can also contribute to remyelination. Using two different inducible myelin-CreER mouse strains in which mature oligodendrocytes were prelabeled by the expression of membrane-bound Green fluorescent protein, we found that after focal spinal cord demyelination, the surrounding surviving labeled oligodendrocytes did not proliferate but remained at a consistent density. Furthermore, existing (prelabeled) oligodendrocytes showed no evidence of incorporation or migration into the lesioned area, or of process extension from the peripheral margins into the lesion. Thus, mature oligodendrocytes do not normally contribute to remyelination and are therefore not a promising target for regenerative therapy.

Citation

Crawford, A. H., Tripathi, R. B., Foerster, S., McKenzie, I., Kougioumtzidou, E., Grist, M., Richardson, W. D., & Franklin, R. J. (2017). Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination. American Journal of Pathology, 186(3), 511-6. https://doi.org/10.1016/j.ajpath.2015.11.005

Journal Article Type	Article
Acceptance Date	Nov 3, 2015
Publication Date	Jan 7, 2017
Deposit Date	Aug 23, 2018
Publicly Available Date	Aug 30, 2018
Journal	American Journal of Pathology
Print ISSN	0002-9440
Electronic ISSN	1525-2191
Publisher	American Society for Investigative Pathology (ASIP)
Peer Reviewed	Peer Reviewed
Volume	186
Issue	3
Pages	511-6
DOI	https://doi.org/10.1016/j.ajpath.2015.11.005
Public URL	https://rvc-repository.worktribe.com/output/1393955