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Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma

Giuliano, A; Swift, R; Arthurs, C; Marote, G; Abramo, F; McKay, J; Thomson, C; Beltran, M; Millar, M; Priestnall, S L; Dobson, J; Costantino-Casas, F; Petrou, T; McGonnell, I M; Davies, A J; Weetman, M; Garden, O A; Masters, J R; Thrasivoulou, C; Ahmed, A


A Giuliano

R Swift

C Arthurs

G Marote

F Abramo

J McKay

C Thomson

M Beltran

M Millar

S L Priestnall

J Dobson

F Costantino-Casas

T Petrou

I M McGonnell

A J Davies

M Weetman

O A Garden

J R Masters

C Thrasivoulou

A Ahmed


Feline oral squamous cell carcinoma (FOSCC) is an aggressive neoplasm in cats. Little is known about the possible molecular mechanisms that may be involved in the initiation, maintenance and progression of FOSCC. Wnt signalling is critical in development and disease, including many mammalian cancers. In this study, we have investigated the expression of Wnt signalling related proteins using quantitative immunohistochemical techniques on tissue arrays. We constructed tissue arrays with 58 individual replicate tissue samples. We tested for the expression of four key Wnt/ß-catenin transcription targets, namely Cyclin D1 (CCND1 or CD1), FRA1, c-Myc and MMP7. All antibodies showed cross reactivity in feline tissue except MMP7. Quantitative immunohistochemical analysis of single proteins (expressed as area fraction / amount of tissue for normal vs tumor, mean ± SE) showed that the expression of CD1 (3.9 ± 0.5 vs 12.2 ± 0.9), FRA1 (5.5 ± 0.6 vs 16.8 ± 1.1) and c-Myc (5.4 ± 0.5 vs 12.5 ± 0.9) was increased in FOSCC tissue by 2.3 to 3 fold compared to normal controls (p


Giuliano, A., Swift, R., Arthurs, C., Marote, G., Abramo, F., McKay, J., …Ahmed, A. (2016). Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma. PLoS ONE, 11(8), e0161103.

Journal Article Type Article
Acceptance Date Jul 26, 2016
Publication Date Aug 25, 2016
Deposit Date Jul 4, 2018
Publicly Available Date Jul 4, 2018
Journal PLoS One
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 11
Issue 8
Pages e0161103
Public URL


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