Skinned fibres produce the same power and force as intact fibre bundles from muscle of wild rabbits

Curtin, N A; Diack, R A; West, T G; Wilson, A M; Woledge, R C

Abstract

Skinned fibres have advantages for comparing the muscle properties of different animal species because they can be prepared from a needle biopsy taken under field conditions. However, it is not clear how well the contractile properties of skinned fibres reflect the properties of the muscle fibres in vivo. Here, we compare the mechanical performance of intact fibre bundles and skinned fibres from muscle of the same animals. This is the first such direct comparison. Maximum power and isometric force were measured at 25°C using peroneus longus (PL) and extensor digiti-V (ED-V) muscles from wild rabbits (Oryctolagus cuniculus). More than 90% of the fibres in these muscles are fast-twitch, type 2 fibres. Maximum power was measured in force-clamp experiments. We show that maximum power per volume was the same in intact (121.3±16.1 W l−1, mean±s.e.m.; N=16) and skinned (122.6±4.6 W l−1; N=141) fibres. Maximum relative power (power/FIM Lo, where FIM is maximum isometric force and Lo is standard fibre length) was also similar in intact (0.645±0.037; N=16) and skinned (0.589±0.019; N=141) fibres. Relative power is independent of volume and thus not subject to errors in measurement of volume. Finally, maximum isometric force per cross-sectional area was also found to be the same for intact and skinned fibres (181.9 kPa±19.1; N=16; 207.8 kPa±4.8; N=141, respectively). These results contrast with previous measurements of performance at lower temperatures where skinned fibres produce much less power than intact fibres from both mammals and non-mammalian species.

Citation

Curtin, N. A., Diack, R. A., West, T. G., Wilson, A. M., & Woledge, R. C. (2015). Skinned fibres produce the same power and force as intact fibre bundles from muscle of wild rabbits. Journal of Experimental Biology, 218, 2856-2863. https://doi.org/10.1242/jeb.121897

• Journal Article Type: Article
• Acceptance Date: Jul 7, 2015
• Publication Date: Sep 23, 2015
• Deposit Date: Aug 12, 2015
• Publicly Available Date: Nov 21, 2020
• Journal: JOURNAL OF EXPERIMENTAL BIOLOGY
• Print ISSN: 0022-0949
• Publisher: The Company of Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 218
• Pages: 2856-2863
• DOI: https://doi.org/10.1242/jeb.121897
• Public URL: https://rvc-repository.worktribe.com/output/1399700