Lipopolysaccharide and toll-like receptor 4 in dogs with congenital portosystemic shunts

Tivers, M S; Lipscomb, V J; Smith, K C; Wheeler-Jones, C P D; House, A K

Abstract

Surgical attenuation of a congenital portosystemic shunt (CPSS) results in increased portal vein perfusion, liver growth and clinical improvement. Portal lipopolysaccharide (LPS) is implicated in liver regeneration via toll-like receptor (TLR) 4 mediated cytokine activation. The aim of this study was to investigate factors associated with LPS in dogs with CPSS. Plasma LPS concentrations were measured in the peripheral and portal blood using a limulus amoebocyte lysate (LAL) assay.

LPS concentration was significantly greater in the portal blood compared to peripheral blood in dogs with CPSS (P = 0.046) and control dogs (P = 0.002). LPS concentrations in the peripheral (P = 0.012) and portal (P = 0.005) blood of dogs with CPSS were significantly greater than those of control dogs. The relative mRNA expression of cytokines and TLRs was measured in liver biopsies from dogs with CPSS using quantitative PCR. TLR4 expression significantly increased following partial CPSS attenuation (P = 0.020). TLR4 expression was significantly greater in dogs that tolerated complete CPSS attenuation (P = 0.011) and those with good portal blood flow on pre-attenuation (P = 0.004) and post-attenuation (P = 0.015) portovenography. Serum interleukin (IL)-6 concentration was measured using a canine specific ELISA and significantly increased 24 h following CPSS attenuation (P 

Citation

Tivers, M. S., Lipscomb, V. J., Smith, K. C., Wheeler-Jones, C. P. D., & House, A. K. (2015). Lipopolysaccharide and toll-like receptor 4 in dogs with congenital portosystemic shunts. https://doi.org/10.1016/j.tvjl.2015.07.006

• Journal Article Type: Article
• Acceptance Date: Jul 1, 2015
• Publication Date: Jul 7, 2015
• Deposit Date: Feb 4, 2016
• Publicly Available Date: Feb 4, 2016
• Journal: VETERINARY JOURNAL
• Peer Reviewed: Peer Reviewed
• Volume: 206
• Issue: 3
• Pages: 404-413
• DOI: https://doi.org/10.1016/j.tvjl.2015.07.006
• Public URL: https://rvc-repository.worktribe.com/output/1400294