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Development and Testing of a Field Diagnostic Assay for Peste des Petits Ruminants Virus

Baron, J; Fishbourne, E; Couacy-Hyman, E; Abubakar, M; Jones, B A; Frost, L; Herbert, R; Chibssa, T R; Van'T Klooster, G; Afzal, M; Ayebazibwe, C; Toye, P; Bashiruddin, J; Baron, M D

Authors

J Baron

E Fishbourne

E Couacy-Hyman

M Abubakar

B A Jones

L Frost

R Herbert

T R Chibssa

G Van'T Klooster

M Afzal

C Ayebazibwe

P Toye

J Bashiruddin

M D Baron



Abstract

We have developed an immunochromatographic test for the diagnosis of peste des petits ruminants (PPR) under field conditions. The diagnostic assay has been tested in the laboratory and also under field conditions in Ivory Coast, Pakistan, Ethiopia and Uganda. The test is carried out on a superficial swab sample (ocular or nasal) and showed a sensitivity of 84% relative to PCR. The specificity was 95% over all nasal and ocular samples. The test detected as little as 103 TCID50 (50% tissue culture infectious doses) of cell culture‐grown virus, and detected virus isolates representing all four known genetic lineages of peste des petits ruminants virus. Virus could be detected in swabs from animals as early as 4 days post‐infection, at a time when clinical signs were minimal. Feedback from field trials was uniformly positive, suggesting that this diagnostic tool may be useful for current efforts to control the spread of PPR.

Citation

Baron, J., Fishbourne, E., Couacy-Hyman, E., Abubakar, M., Jones, B. A., Frost, L., …Baron, M. D. (2014). Development and Testing of a Field Diagnostic Assay for Peste des Petits Ruminants Virus. Transboundary and Emerging Diseases, 61(5), 390-396. https://doi.org/10.1111/tbed.12266

Journal Article Type Article
Acceptance Date Jul 1, 2014
Publication Date Jul 30, 2014
Deposit Date Nov 12, 2014
Publicly Available Date Feb 15, 2019
Journal Transboundary and Emerging Diseases
Print ISSN 1865-1674
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 61
Issue 5
Pages 390-396
DOI https://doi.org/10.1111/tbed.12266
Public URL https://rvc-repository.worktribe.com/output/1405158
Additional Information Corporate Creators : FAO, ILRI, NADDEC, NAHDIC, NVL, Pakistan, Pirbright, Virology Lab, Ivory Coast

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