MicroRNAs control the apoptotic threshold in primed Pluripotent stem cells through regulation of BIM

Pernaute, B; Spruce, T; Smith, K M; Sanchez-Nieto, J M; Manzanares, M; Cobb, B S; Rodriguez, T A

Abstract

Mammalian primed pluripotent stem cells have been shown to be highly susceptible to cell death stimuli due to their low apoptotic threshold, but how this threshold is regulated remains largely unknown. Here we identify microRNA (miRNA)-mediated regulation as a key mechanism controlling apoptosis in the post-implantation epiblast. Moreover, we found that three miRNA families, miR-20, miR-92, and miR-302, control the mitochondrial apoptotic machinery by fine-tuning the levels of expression of the proapoptotic protein BIM. These families therefore represent an essential buffer needed to maintain cell survival in stem cells that are primed for not only differentiation but also cell death.

Citation

Pernaute, B., Spruce, T., Smith, K. M., Sanchez-Nieto, J. M., Manzanares, M., Cobb, B. S., & Rodriguez, T. A. (in press). MicroRNAs control the apoptotic threshold in primed Pluripotent stem cells through regulation of BIM. https://doi.org/10.1101/gad.245621.114

• Journal Article Type: Article
• Acceptance Date: Jul 24, 2014
• Deposit Date: Nov 11, 2014
• Publicly Available Date: Sep 17, 2018
• Journal: Genes & Development
• Peer Reviewed: Peer Reviewed
• Volume: 28
• Issue: 17
• Pages: 1873-1878
• DOI: https://doi.org/10.1101/gad.245621.114
• Public URL: https://rvc-repository.worktribe.com/output/1405193