Anti-Insulin Antibodies in Diabetic Dogs Before and After Treatment with Different Insulin Preparations

Davison, L J; Walding, B R; Herrtage, M E; Catchpole, B

Abstract

Background: Anti-insulin antibodies (AIA) occur in diabetic dogs after insulin therapy, although their clinical significance is unclear. Hypothesis: Treatment of diabetic dogs with heterologous insulin is more likely to stimulate production of AIA than is treatment with homologous insulin. Animals: Diabetic dogs sampled before insulin therapy (n = 40), diabetic dogs sampled following treatment with porcine (homologous) insulin (n = 100), bovine (heterologous) lente insulin (n = 100), or bovine protamine zinc (PZI) insulin (n = 20), and nondiabetic control dogs (n = 120). Methods: Prospective observational study. Sera were analyzed by ELISA for antibodies against porcine insulin, bovine insulin, insulin A, B, or C peptides, and control antigens; canine distemper virus (CDV) and canine thyroglobulin (TG). Canine isotype-specific antibodies were used to determine total and anti-insulin IgG1 : IgG2 ratios. Results: There was no difference in CDV or TG reactivity among the groups. AIA were detected in 5 of 40 newly diagnosed (untreated) diabetic dogs. There was no significant difference in AIA (ELISA optical density reactivity) comparing control and porcine insulin-treated diabetic dogs (P > .05). Anti-insulin reactivity was most prevalent in bovine PZI insulin-treated dogs (90%; P < .01), and bovine lente insulin-treated dogs (56%; P < .01). AIA induced by treatment were enriched for the IgG1 isotype. Conclusions and Clinical Importance: This study indicates that bovine insulin is more immunogenic than porcine insulin when used for treatment of diabetic dogs.

Citation

Davison, L. J., Walding, B. R., Herrtage, M. E., & Catchpole, B. Anti-Insulin Antibodies in Diabetic Dogs Before and After Treatment with Different Insulin Preparations. Journal of Veterinary Internal Medicine, 22(6), 1317-1325. https://doi.org/10.1111/j.1939-1676.2008.0194.x

• Journal Article Type: Article
• Deposit Date: Nov 12, 2014
• Journal: Journal of Veterinary Internal Medicine
• Print ISSN: 0891-6640
• Electronic ISSN: 1939-1676
• Publisher: Wiley Open Access
• Volume: 22
• Issue: 6
• Pages: 1317-1325
• DOI: https://doi.org/10.1111/j.1939-1676.2008.0194.x
• Public URL: https://rvc-repository.worktribe.com/output/1429089
• Additional Information: Corporate Creators : Cambridge