Differences in the proportion of collagen and muscle in the canine lower urinary tract with regard to gonadal status and gender

Ponglowhapan, S; Church, D B; Khalid, M

Abstract

Gonadectomy not only affects hormonal homeostasis but also alters the turnover of different components of the extracellular matrix in urogenital tissues. Collagen is an important component of the bladder and urethral walls and thus crucial for the mechanical properties of normal lower urinary tract (LUT) functions. This study aimed at investigating the possibility of differences in the proportion of collagen and muscle tissues in the LUT of intact and gonadectomised male and female dogs. Twenty clinically healthy dogs were used including 10 sexually intact dogs (5 males, 5 anoestrus females) and 10 gonadectomised dogs (4 males and 6 females). Four regions of the LUT, i.e. body and neck of the bladder as well as proximal and distal urethra were collected. The tissue sections were stained with Masson's Trichrome. Quantitative evaluation of the blue-stained area for collagen and red-counterstained area for muscle was performed using colour image analysis. The relative proportion of collagen and muscle significantly differed with the gonadal status, the gender and the region. Overall, gonadectomised dogs had a higher (P

Citation

Ponglowhapan, S., Church, D. B., & Khalid, M. Differences in the proportion of collagen and muscle in the canine lower urinary tract with regard to gonadal status and gender. Theriogenology, 70(9), 1516-1524. https://doi.org/10.1016/j.theriogenology.2008.06.099

• Journal Article Type: Article
• Deposit Date: Nov 12, 2014
• Journal: Theriogenology
• Print ISSN: 0093-691X
• Publisher: Elsevier
• Volume: 70
• Issue: 9
• Pages: 1516-1524
• DOI: https://doi.org/10.1016/j.theriogenology.2008.06.099
• Public URL: https://rvc-repository.worktribe.com/output/1430637