Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos

Stramer, B; Winfield, M; Shaw, T; Millard, T H; Woolner, S; Martin, P

Abstract

By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.

Citation

Stramer, B., Winfield, M., Shaw, T., Millard, T. H., Woolner, S., & Martin, P. Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos. EMBO Reports, 9(5), 465-471. https://doi.org/10.1038/embor.2008.34

• Journal Article Type: Article
• Deposit Date: Nov 11, 2014
• Journal: EMBO Reports
• Print ISSN: 1469-221X
• Electronic ISSN: 1469-3178
• Publisher: Wiley
• Volume: 9
• Issue: 5
• Pages: 465-471
• DOI: https://doi.org/10.1038/embor.2008.34
• Public URL: https://rvc-repository.worktribe.com/output/1431143
• Additional Information: Corporate Creators : Bristol, Wisconsin