Analysis of variation of bovine viral diarrhoea virus E2 sequence following transplacental infection of cattle

Stokstad, M; Brownlie, J; Collins, M E

Abstract

The genetic and antigenic diversity observed in field isolates of bovine viral diarrhoea virus (BVDV) is thought to occur during acute infection because of the genetic stability observed in BVDV throughout the lifetime of persistently infected (PI) cattle. In this study, 15 cows in early pregnancy were inoculated with identical challenge doses obtained from a single infectious inoculum of the virologically cloned isolate Pe515nc. In order to examine the diversity that may develop in utero in the PI foetus, the variable E2 sequence of the virus isolated directly from the serum of each PI calf was compared. A high degree of sequence similarity was demonstrated, with 0–4 nucleotide differences out of 608 bases compared. Thus, the virus showed relatively few genomic changes in any of the PI calves, although we observed that the in utero environment did provide some opportunity for genetic variation to become established.

Citation

Stokstad, M., Brownlie, J., & Collins, M. E. (2004). Analysis of variation of bovine viral diarrhoea virus E2 sequence following transplacental infection of cattle. Veterinary Microbiology, 102(3-4), 141-145. https://doi.org/10.1016/j.vetmic.2004.06.015

• Journal Article Type: Article
• Acceptance Date: Jun 9, 2004
• Publication Date: Aug 14, 2004
• Deposit Date: Nov 11, 2014
• Journal: VETERINARY MICROBIOLOGY
• Print ISSN: 0378-1135
• Publisher: Elsevier
• Volume: 102
• Issue: 3-4
• Pages: 141-145
• DOI: https://doi.org/10.1016/j.vetmic.2004.06.015
• Public URL: https://rvc-repository.worktribe.com/output/1435934
• Additional Information: Corporate Creators : Norwegian School of Vet Science, Oslo