PROSPECTIVE EVALUATION OF PLATELET FUNCTION AND FIBRINOLYSIS IN 20 DOGS WITH TRAUMA

Cortellini, Stefano; Chan, Daniel; McBride, Duana; Birkbeck, Rachael

Abstract

Objectives: To determine platelet function and fibrinolysis in dogs following trauma using multiple electrical impedance aggregometry and a modified thromboelastographic technique. To determine if the severity of trauma, as assessed by the Animal Trauma Triage score and clinicopathological markers of shock, is associated with a greater degree of platelet dysfunction and fibrinolysis.

Methods: Twenty client-owned dogs with trauma (occurring 4 were prospectively recruited. A control group of 10 healthy dogs were included. Platelet function was measured using multiple electrical impedance aggregometry (MEPA) utilising arachidonic acid (AA), adenosine diphosphate (ADP) and collagen (COL) agonists. Fibrinolysis was assessed in citrated whole blood with the addition of tissue plasminogen activator (tPA 50U/ml) using kaolin-activated thromboelastography. Conventional statistical analysis was performed to compare coagulation parameters between the groups and assess linear correlations.

Results: The median (IQR) ATT score was 5 (5-7) and 65% (n=13) of dogs suffered polytrauma. The mean (±SD) time from trauma to blood sampling was 9 hours (±6). Shock index and plasma lactate concentration were 1.1 (1.3, n=16) and 2.9 mmol/L (1.9 n=17), respectively. Four dogs did not survive to discharge (20%). There was no difference between the trauma and control group coagulation variables. A moderate negative correlation between ATT score and AUC for ADP was found.

Clinical significance: Platelet function measured by MEPA and fibrinolysis measured by TEG is not significantly different in this population of dogs with traumatic injury compared to healthy dogs.

Citation

Cortellini, S., Chan, D., McBride, D., & Birkbeck, R. (in press). PROSPECTIVE EVALUATION OF PLATELET FUNCTION AND FIBRINOLYSIS IN 20 DOGS WITH TRAUMA. Journal of Veterinary Emergency and Critical Care, https://doi.org/10.1111/vec.13356

• Journal Article Type: Article
• Acceptance Date: Sep 24, 2022
• Online Publication Date: Dec 6, 2023
• Deposit Date: Dec 8, 2023
• Publicly Available Date: Dec 8, 2023
• Journal: Journal of Veterinary Emergency and Critical Care
• Print ISSN: 1479-3261
• Electronic ISSN: 1476-4431
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1111/vec.13356