V Bergomi
Insulin expression in beta cells is reduced within islets before islet loss in diabetic cats
Bergomi, V; Beck, S; Dobromylskya, M; Davison, LJ; Wills, JW; Hughes, K
Authors
S Beck
M Dobromylskya
LJ Davison
JW Wills
K Hughes
Abstract
Objectives Diabetes mellitus is a common condition that requires intensive treatment and markedly impacts the welfare of affected cats. The aim of this study was to identify diabetes mellitus-associated perturbations in the feline pancreatic islet microenvironment. The utility of "clear, unobstructed brain/body imaging cocktails and computational analysis" (CUBIC) for three-dimensional pancreatic analysis was investigated. Methods Formalin-fixed paraffin-embedded tissues from cats with diabetes mellitus, or control cats without pancreatic pathology, were retrospectively identified. Immunohistochemistry for synaptophysin and ionised calcium binding adaptor molecule 1, and immunofluorescence for insulin and synaptophysin, were used to assess changes in islets. An image analysis pipeline was developed to analyse images acquired from two-dimensional immunofluorescence. CUBIC was used to optically clear selected pancreas samples before immunofluorescence and deep three-dimensional confocal microscopy. Results Diabetic cats have a significant reduction in synaptophysin-positive islet area. Whilst islets from diabetic patients have similar numbers of beta cells to islets from control cats, significantly lower intensity of insulin expression can be observed in the former. CUBIC facilitates clear visualisation of pancreatic islets in three dimensions. Clinical Significance The data presented support the theory that there is a decrease in function of beta cells before their destruction, suggesting a potentially significant step in the pathogenesis of feline diabetes mellitus. In parallel, we demonstrate CUBIC as a valuable new tool to visualise the shape of feline pancreatic islets and to interrogate pathology occurring in the islets of diabetic pets.
Citation
Bergomi, V., Beck, S., Dobromylskya, M., Davison, L., Wills, J., & Hughes, K. (in press). Insulin expression in beta cells is reduced within islets before islet loss in diabetic cats. Journal of Small Animal Practice, https://doi.org/10.1111/jsap.13541
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 7, 2022 |
Online Publication Date | Aug 19, 2022 |
Deposit Date | May 16, 2024 |
Publicly Available Date | May 16, 2024 |
Print ISSN | 0022-4510 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1111/jsap.13541 |
Keywords | URINARY-TRACT-INFECTIONS; RISK-FACTORS; HUMANS; KETOACIDOSIS; PATHOGENESIS; MELLITUS; OBESITY; MODELS; MASS |
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Publisher Licence URL
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