Serum 1H nuclear magnetic resonance-based metabolomics of sole lesion development in Holstein cows

Barden, M; Phelan, MM; Hyde, RB; Anagnostopoulos, A; Griffiths, BE; Bedford, C; Green, M; Psifidi, A; Banos, G; Oikonomou, G

Abstract

those with the strongest evidence of being associated with sole lesions corresponded to phenylalanine and 4 unlabeled metabolites. We conclude that the serum metabolome, as characterized by proton nuclear magnetic resonance spectroscopy, does not appear able to predict sole lesion presence or future development of lesions. A small number of metabolites may be associated with sole lesions although, given the poor prediction accuracies, these metabolites are likely to explain only a small proportion of the differences between affected and unaffected animals. Future metabolomic studies may reveal underlying metabolic mechanisms of sole lesion etiopathogenesis in dairy cows; however, the experimental design and analysis need to effectively control for interanimal and extraneous sources of spectral variation.

Citation

Barden, M., Phelan, M., Hyde, R., Anagnostopoulos, A., Griffiths, B., Bedford, C., Green, M., Psifidi, A., Banos, G., & Oikonomou, G. (2023). Serum 1H nuclear magnetic resonance-based metabolomics of sole lesion development in Holstein cows. Journal of Dairy Science, 106(4), 2667-2684. https://doi.org/10.3168/jds.2022-22681

• Journal Article Type: Article
• Acceptance Date: Nov 15, 2022
• Online Publication Date: Mar 2, 2023
• Publication Date: 2023
• Deposit Date: Feb 6, 2024
• Publicly Available Date: Feb 6, 2024
• Print ISSN: 0022-0302
• Electronic ISSN: 1525-3198
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 106
• Issue: 4
• Pages: 2667-2684
• DOI: https://doi.org/10.3168/jds.2022-22681
• Keywords: lameness; claw horn lesions; metabolomics; nuclear magnetic resonance (NMR) spectroscopy; WHITE LINE DISEASE; DAIRY-CATTLE; RISK-FACTORS; CLAW LESIONS; FOOT LESIONS; LAMENESS; ULCERS; PREVALENCE; HEIFERS; PROFILE