Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence <i>Eimeria tenella</i> Intracellular Development

Felici, M; Tugnoli, B; De Hoest-Thompson, C; Piva, A; Grilli, E; Marugan-Hernandez, V

Abstract

Coccidiosis poses a significant challenge in poultry production and is typically managed with ionophores and chemical anticoccidials. However, the emergence of drug resistance and limitations on their use have encouraged the exploration of alternative solutions, including botanical compounds and improvements in in vitro screening methods. Prior research focused only on the impact of these alternatives on Eimeria invasion, with intracellular development in cell cultures receiving limited attention. This study assessed the impact of thyme (Thymus vulgaris), oregano (Origanum vulgare), and garlic (Allium sativum) essential oils, as well as their bioactive compounds, on the initial phase of schizogony in Madin-Darby bovine kidney cells, comparing their effectiveness to two commercially used anticoccidial drugs. Using image analysis and quantitative PCR, the study confirmed the efficacy of commercial anticoccidials in reducing invasion and schizont formation, and it found that essential oils were equally effective. Notably, thymol and carvacrol exhibited mild inhibition of intracellular replication of the parasite but significantly reduced schizont numbers, implying a potential reduction in pathogenicity. In conclusion, this research highlights the promise of essential oils and their bioactive components as viable alternatives to traditional anticoccidial drugs for mitigating coccidiosis in poultry, particularly by disrupting the intracellular development of the parasites.

Citation

Felici, M., Tugnoli, B., De Hoest-Thompson, C., Piva, A., Grilli, E., & Marugan-Hernandez, V. (2024). Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence Eimeria tenella Intracellular Development. Animals, 14(1), https://doi.org/10.3390/ani14010077

• Journal Article Type: Article
• Acceptance Date: Dec 23, 2023
• Online Publication Date: Dec 25, 2023
• Publication Date: 2024
• Deposit Date: Jan 16, 2024
• Publicly Available Date: Jan 16, 2024
• Print ISSN: 2076-2615
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 1
• DOI: https://doi.org/10.3390/ani14010077
• Keywords: avian coccidiosis; Eimeria tenella; in vitro; schizogony; botanicals; anticoccidials; ALLIUM-SATIVUM; CULTURE; SYSTEMS