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Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12
Journal Article
Bossé, J. T., Li, Y., Angen, Ø., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., …Langford, P. R. (in press). Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12. Journal of Clinical Microbiology, 52, 2380-2385. https://doi.org/10.1128/JCM.00685-14

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative am... Read More about Multiplex PCR assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10 and 12.

Pharmacokinetic/pharmacodynamic integration and modelling of amoxicillin for the calf pathogens Mannheimia haemolytica and Pasteurella multocida
Journal Article
Lees, P., Pelligand, L., Illambas, J., Potter, T. J., Lacroix, M., Rycroft, A. N., & Toutain, P. L. (in press). Pharmacokinetic/pharmacodynamic integration and modelling of amoxicillin for the calf pathogens Mannheimia haemolytica and Pasteurella multocida. Journal of Veterinary Pharmacology and Therapeutics, 38(5), 457-70. https://doi.org/10.1111/jvp.12207

The antimicrobial properties of amoxicillin were determined for the bovine respiratory tract pathogens, Mannheima haemolytica and Pasteurella multocida. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time‐kill cu... Read More about Pharmacokinetic/pharmacodynamic integration and modelling of amoxicillin for the calf pathogens Mannheimia haemolytica and Pasteurella multocida.

Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis
Journal Article
Weinert, L. A., Chaudhuri, R. R., Wang, J., Peters, S. E., Corander, J., Jombart, T., …BRaDP1T Consortium, . (in press). Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis. Nature Communications, 6, https://doi.org/10.1038/ncomms7740

Streptococcus suis causes disease in pigs worldwide and is increasingly implicated in zoonotic disease in East and South-East Asia. To understand the genetic basis of disease in S. suis, we study the genomes of 375 isolates with detailed clinical phe... Read More about Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis.

Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae
Journal Article
Bossé, J. T., Li, Y., Atherton, T. G., Walker, S., Williamson, S. M., Rogers, J., …Langford, P. R. (in press). Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae. Veterinary Microbiology, 178(3-4), 279-282. https://doi.org/10.1016/j.vetmic.2015.05.020

The complete nucleotide sequence of a 7.7 kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae contain... Read More about Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae.

CORRIGENDUM TO 'CHARACTERISATION OF A MOBILISABLE PLASMID CONFERRING FLORFENICOL AND CHLORAMPHENICOL RESISTANCE IN ACTINOBACILLUS PLEUROPNEUMONIAE' [VOL 178, PG 279, 2015]
Journal Article
Bossé, J. T., Li, Y., Atherton, T. G., Walker, S., Williamson, S. M., Rogers, J., …BRaDP1T Consortium, . (in press). CORRIGENDUM TO 'CHARACTERISATION OF A MOBILISABLE PLASMID CONFERRING FLORFENICOL AND CHLORAMPHENICOL RESISTANCE IN ACTINOBACILLUS PLEUROPNEUMONIAE' [VOL 178, PG 279, 2015]. Veterinary Microbiology, 179(3-4), 341-341. https://doi.org/10.1016/j.vetmic.2015.07.002

Generation and evaluation of a Glaesserella (Haemophilus) parasuis capsular mutant
Journal Article
Eberle, K. C., Hau, S. J., Luan, S., Weinert, L. A., Stasko, J. A., Wang, J., …Brockmeier, S. L. (in press). Generation and evaluation of a Glaesserella (Haemophilus) parasuis capsular mutant. Infection and Immunity, https://doi.org/10.1128/IAI.00879-19

Glaesserella (Haemophilus) parasuis is a commensal of the upper respiratory tract in pigs and also the causative agent of Glässer's disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serova... Read More about Generation and evaluation of a Glaesserella (Haemophilus) parasuis capsular mutant.

Aorto-iliac thromboembolism secondary to infectious endocarditis causing paraparesis in a puppy
Journal Article
Penning, V., Benigni, L., Connolly, D. J., Chandler, K. E., Smyth, B., Rycroft, A. N., & Whatmough, C. Aorto-iliac thromboembolism secondary to infectious endocarditis causing paraparesis in a puppy. Companion Animal, 13(7), 57-62. https://doi.org/10.1111/j.2044-3862.2008.tb00317.x

A 10-week-old-female Rhodesian Ridgeback was referred for right pelvic limb lameness following a fall. A grade IV/VI left atrioventricular (AV) valve murmur and reduced femoral pulses were detected. On neurological examination the puppy was non-ambul... Read More about Aorto-iliac thromboembolism secondary to infectious endocarditis causing paraparesis in a puppy.

Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae
Journal Article
Oldfield, N. J., Donovana, E. A., Worrall, K. E., Wooldridge, K. G., Langford, P. R., Rycroft, A. N., & Ala'Aldeen, D. A. A. Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae. Vaccine, 26(16), 1942-1954. https://doi.org/10.1016/j.vaccine.2008.02.022

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine com... Read More about Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae.

Novel adhesin from Pasteurella multocida that binds to the integrin-binding fibronectin FnIII(9-10) repeats
Journal Article
Mullen, L. M., Nair, S. P., Ward, J. M., Rycroft, A. N., Williams, R. J., Robertson, G., …Henderson, B. Novel adhesin from Pasteurella multocida that binds to the integrin-binding fibronectin FnIII(9-10) repeats. Infection and Immunity, 76(3), 1093-1104. https://doi.org/10.1128/iai.01349-07

Phage display screening with fragmented genomic DNA from the animal pathogen Pasteurella multocida has identified a gene encoding a putative fibronectin binding protein (19). Homologues of this gene (PM1665) are found in all other sequenced members o... Read More about Novel adhesin from Pasteurella multocida that binds to the integrin-binding fibronectin FnIII(9-10) repeats.

Multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains
Journal Article
Zhou, L., Jones, S. C. P., Angen, Ø., Bossé, J. T., Nash, J. H. E., Frey, J., …Langford, P. R. Multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains. Journal of Clinical Microbiology, 46(2), 800-803. https://doi.org/10.1128/jcm.01787-07

We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in convention... Read More about Multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.

PCR specific for Actinobacillus pleuropneumoniae serotype 3
Journal Article
Zhou, L., Jones, S. C. P., Angen, Ø., Bossé, J. T., Nash, J. H. E., Frey, J., …Langford, P. R. PCR specific for Actinobacillus pleuropneumoniae serotype 3. Veterinary Record, 162(20), 648-+

Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivit... Read More about PCR specific for Actinobacillus pleuropneumoniae serotype 3.