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All Outputs (203)

Porcupine inhibition is a promising pharmacological treatment for severe sclerosteosis pathologies (2025)
Journal Article
Dreyer, T., Keen, J., Wells, L., Hopkinson, M., Orriss, I., Holdsworth, G., Pitsillides, A., & Roberts, S. (2025). Porcupine inhibition is a promising pharmacological treatment for severe sclerosteosis pathologies. Bone Research, 13(1), https://doi.org/10.1038/s41413-025-00406-3

Sclerosteosis, an ultra-rare disorder characterised by high bone mass (HBM) and skeletal overgrowth, leads to facial paralysis, hearing loss and raised intracranial pressure, which is currently managed only through high-risk surgery. Sclerosteosis is... Read More about Porcupine inhibition is a promising pharmacological treatment for severe sclerosteosis pathologies.

Extracellular matrix: Dystroglycan interactions-Roles for the dystrophin-associated glycoprotein complex in skeletal tissue dynamics (2025)
Journal Article
Hopkinson, M., & Pitsillides, A. (2025). Extracellular matrix: Dystroglycan interactions-Roles for the dystrophin-associated glycoprotein complex in skeletal tissue dynamics. International Journal of Experimental Pathology, 106(2), https://doi.org/10.1111/iep.12525

Contributions made by the dystrophin-associated glycoprotein complex (DGC) to cell-cell and cell-extracellular matrix (ECM) interactions are vital in development, homeostasis and pathobiology. This review explores how DGC functions may extend to skel... Read More about Extracellular matrix: Dystroglycan interactions-Roles for the dystrophin-associated glycoprotein complex in skeletal tissue dynamics.

Autoimmunity to stromal-derived autoantigens in rheumatoid ectopic germinal centers exacerbates arthritis and affects clinical response (2024)
Journal Article
Corsiero, E., Caliste, M., Jagemann, L., Fossati-Jimack, L., Goldmann, K., Cubuk, C., Ghirardi, G., Prediletto, E., Rivellese, F., Alessandri, C., Hopkinson, M., Javaheri, B., Pitsillides, A., Lewis, M., Pitzalis, C., & Bombardieri, M. (2024). Autoimmunity to stromal-derived autoantigens in rheumatoid ectopic germinal centers exacerbates arthritis and affects clinical response. Journal of Clinical Investigation, 134(12), https://doi.org/10.1172/JCI169754

Ectopic lymphoid structures (ELSs) in the rheumatoid synovial joints sustain autoreactivity against locally expressed autoantigens. We recently identified recombinant monoclonal antibodies (RA-rmAbs) derived from single, locally differentiated rheuma... Read More about Autoimmunity to stromal-derived autoantigens in rheumatoid ectopic germinal centers exacerbates arthritis and affects clinical response.

Expression of semaphorin-3A in the joint and role in osteoarthritis (2024)
Journal Article
Li, X., Martinez-Ramos, S., Heedge, F., Pitsillides, A., Bou-Gharios, G., Poulet, B., & Chenu, C. (2024). Expression of semaphorin-3A in the joint and role in osteoarthritis. Cell Biochemistry and Function, 42(3), https://doi.org/10.1002/cbf.4012

Osteoarthritis (OA) is characterised by the deterioration of cartilage in the joints and pain. We hypothesise that semaphorin-3A (sema-3A), a chemorepellent for sensory nerves, plays a role in joint degradation and pain. We used the mechanical joint... Read More about Expression of semaphorin-3A in the joint and role in osteoarthritis.

Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis (2024)
Journal Article
Louka, P., Orriss, I. R., & Pitsillides, A. A. (2024). Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis. Cells, 13(2), https://doi.org/10.3390/cells13020165

Sulforaphane, the native but unstable form of SFX-01, is an antioxidant that activates the NRF2 and inhibits the NF-KB pathways to achieve its actions. Resolving the mechanism(s) by which SFX-01 serves to control the various osteoclastogenic stages m... Read More about Stable Sulforaphane Targets the Early Stages of Osteoclast Formation to Engender a Lasting Functional Blockade of Osteoclastogenesis.

Bone marrow lesions: plugging the holes in our knowledge using animal models (2023)
Journal Article
Hansen, R., Chenu, C., Sofat, N., & Pitsillides, A. (2023). Bone marrow lesions: plugging the holes in our knowledge using animal models. Nature Reviews Rheumatology, https://doi.org/10.1038/s41584-023-00971-z

Bone marrow lesions (BMLs), which are early signs of osteoarthritis (OA) that are associated with the presence, onset and severity of pain, represent an emerging imaging biomarker and clinical target. Little is known, however, regarding their early s... Read More about Bone marrow lesions: plugging the holes in our knowledge using animal models.

Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models (2023)
Journal Article
Salmon, P., Monzem, S., Javaheri, B., Oste, L., Kerckhofs, G., & Pitsillides, A. (2023). Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models. Frontiers in Endocrinology, 14, https://doi.org/10.3389/fendo.2023.1158099

Introduction: Histomorphometry of rodent metaphyseal trabecular bone, by histology or microCT, is generally restricted to the mature secondary spongiosa, excluding the primary spongiosa nearest the growth plate by imposing an `offset'. This analyses... Read More about Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models.

Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice (2023)
Journal Article
Monzem, S., Valkani, D., Evans, L., Chang, Y., & Pitsillides, A. (2023). Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice. BONE, 170, https://doi.org/10.1016/j.bone.2023.116720

Beneficial effects of intermittent parathyroid hormone (PTH) on bone mass and architecture are described to either simply add to, or to synergise with those of mechanical loading. We evaluate whether interaction with in vivo loading is reinforced by... Read More about Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice.

Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation (2022)
Journal Article
Disney, C., Mo, J., Eckersley, A., Bodey, A., Hoyland, J., Sherratt, M., Pitsillides, A., Lee, P., & Bay, B. (2022). Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation. Acta Biomaterialia, 138, 361-374. https://doi.org/10.1016/j.actbio.2021.10.012

Many soft tissues, such as the intervertebral disc (IVD), have a hierarchical fibrous composite structure which suffers from regional damage. We hypothesise that these tissue regions have distinct, inherent fibre structure and structural response upo... Read More about Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation.

A new method for segmentation and analysis of bone callus in rodent fracture models using micro-CT (2022)
Journal Article
Hopkinson, M., Jones, G., Evans, L., Gohin, S., Magnusdottir, R., Salmon, P., Chenu, C., Meeson, R., Javaheri, B., & Pitsillides, A. (2022). A new method for segmentation and analysis of bone callus in rodent fracture models using micro-CT. Journal of Orthopaedic Research, https://doi.org/10.1002/jor.25507

Fracture burden has created a need to better understand bone repair processes under different pathophysiological states. Evaluation of structural and material properties of the mineralized callus, which is integral to restoring biomechanical stabilit... Read More about A new method for segmentation and analysis of bone callus in rodent fracture models using micro-CT.

Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting (2022)
Journal Article
Santos-Beato, P., Midha, S., Pitsillides, A., Miller, A., Torii, R., & Kalaskar, D. (2022). Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting. Journal of Tissue Engineering, 13, https://doi.org/10.1177/20417314221133480

Multiple prevalent diseases, such as osteoarthritis (OA), for which there is no cure or full understanding, affect the osteochondral unit; a complex interface tissue whose architecture, mechanical nature and physiological characteristics are still ye... Read More about Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting.

Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy (2022)
Journal Article
Hsu, S., Stephen, L., Dillon, S., Milne, E., Javaheri, B., Pitsillides, A., Novak, A., Millan, J., MacRae, V., Staines, K., & Farquharson, C. (2022). Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy. Journal of Endocrinology, 254(3), 167-181. https://doi.org/10.1530/JOE-22-0097

Patients with advanced chronic kidney disease (CKD) often present with skeletal abnormalities, a condition known as renal osteodystrophy (ROD). While tissue non-specific alkaline phosphatase (TNAP) and PHOSPHO1 are critical for bone mineralization, t... Read More about Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy.

MCTR3 reprograms arthritic monocytes to upregulate Arginase-1 and exert pro-resolving and tissue-protective functions in experimental arthritis (2022)
Journal Article
Pistorius, K., Ly, L., Souza, P., Gomez, E., Koenis, D., Rodriguez, A., Foster, J., Sosabowski, J., Hopkinson, M., Rajeeve, V., Spur, B., Pitsillides, A., Pitzalis, C., & Dalli, J. (2022). MCTR3 reprograms arthritic monocytes to upregulate Arginase-1 and exert pro-resolving and tissue-protective functions in experimental arthritis. EBioMedicine, 79, https://doi.org/10.1016/j.ebiom.2022.103974

Spatial links between subchondral bone architectural features and cartilage degeneration in osteoarthritic joints (2022)
Journal Article
Ajami, S., Javaheri, B., Chang, Y., Maruthainar, N., Khan, T., Donaldson, J., Pitsillides, A., & Liu, C. (2022). Spatial links between subchondral bone architectural features and cartilage degeneration in osteoarthritic joints. Scientific Reports, 12(1), https://doi.org/10.1038/s41598-022-10600-6

Early diagnosis of osteoarthritis (OA), before the onset of irreversible changes is crucial for understanding the disease process and identifying potential disease-modifying treatments from the earliest stage. OA is a whole joint disease and affects... Read More about Spatial links between subchondral bone architectural features and cartilage degeneration in osteoarthritic joints.

Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis (2022)
Journal Article
Jones, G., Pitsillidies, A., & Meeson, R. (2022). Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2022.789898

Osteoarthritis (OA) is the most common orthopaedic condition in dogs, characterised as chronic, painful end-point of a synovial joint with limited therapeutic options other than palliative pain control or surgical salvage. Since the 1970s, radiograph... Read More about Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis.

Age and Sex Differences in Load-Induced Tibial Cortical Bone Surface Strain Maps (2021)
Journal Article
Carriero, A., Javaheri, B., Kazeruni, N., Pitsillides, A., & Shefelbine, S. (2021). Age and Sex Differences in Load-Induced Tibial Cortical Bone Surface Strain Maps. JBMR Plus, 5(3), https://doi.org/10.1002/jbm4.10467

Bone adapts its architecture to the applied load; however, it is still unclear how bone mechano-adaptation is coordinated and why potential for adaptation adjusts during the life course. Previous animal models have suggested strain as the mechanical... Read More about Age and Sex Differences in Load-Induced Tibial Cortical Bone Surface Strain Maps.

Multiscale molecular profiling of pathological bone resolves sexually dimorphic control of extracellular matrix composition (2021)
Journal Article
Sharma, A., Goring, A., Johnson, P., Emery, R., Hesse, E., Boyde, A., Olsen, B., Pitsillides, A., Oreffo, R., Mahajan, S., & Clarkin, C. (2021). Multiscale molecular profiling of pathological bone resolves sexually dimorphic control of extracellular matrix composition. Disease Models and Mechanisms, 14(3), https://doi.org/10.1242/dmm.048116

Collagen assembly during development is essential for successful matrix mineralisation, which determines bone quality and mechanocompetence. However, the biochemical and structural perturbations that drive pathological skeletal collagen configuration... Read More about Multiscale molecular profiling of pathological bone resolves sexually dimorphic control of extracellular matrix composition.