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All Outputs (95)

Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs (2020)
Journal Article
Bossé, J. T., Li, Y., Fernandez Crespo, R., Angen, Ø., Holden, M. T. G., Weinert, L. A., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., & BRaDP1T Consortium. (2020). Draft Genome Sequences of the Type Strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), Two NAD-Dependent Bacterial Species Found in the Respiratory Tract of Pigs. Microbiology Resource Announcements, 9(1), https://doi.org/10.1128/MRA.00716-19

Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales (2019)
Journal Article
Wileman, T. M., Weinert, L. A., Howell, K. J., Wang, J., Peters, S. E., Williamson, S. M., Wells, J. M., Langford, P. R., Rycroft, A. N., Wren, B. W., Maskell, D. J., & Tucker, A. W. (2019). Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales. Journal of Clinical Microbiology, 57, e01712. https://doi.org/10.1128/JCM.01712-18

Streptococcus suis is one of the most important zoonotic bacterial pathogens of pigs, causing significant economic losses to the global swine industry. S. suis is also a very successful colonizer of mucosal surfaces, and commensal strains can be foun... Read More about Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales.

Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media (2019)
Journal Article
Mead, A., Lees, P., Mitchell, J., Rycroft, A. N., Standing, J. F., Toutain, P. L., & Pelligand, L. (2019). Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media. Journal of Veterinary Pharmacology and Therapeutics, 42(1), 52-59. https://doi.org/10.1111/jvp.12719

For clinical isolates of bovine Mannheimia haemolytica and Pasteurella multocida, this study reports minimum inhibitory concentration (MIC) differences for tetracycline, oxytetracycline and doxycycline between cation‐adjusted Mueller‐Hinton broth (CA... Read More about Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media.

Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing (2018)
Journal Article
Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Amoribieta, M. C., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., & Langford, P. R. (2018). Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing. Veterinary Microbiology, 220, 83-89. https://doi.org/10.1016/j.vetmic.2018.05.011

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were devel... Read More about Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis (2018)
Journal Article
Bossé, J. T., Li, Y., Sárközi, R., Fodor, L., Lacouture, S., Gottschalk, M., Amoribieta, M. C., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., & Langford, P. R. (2018). Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis. Veterinary Microbiology, 217, 1-6. https://doi.org/10.1016/j.vetmic.2018.02.019

The aim of this study was to investigate isolates of Actinobacillus pleuropneumoniae previously designated serologically either as NT or as ‘K2:07’, which did not produce serovar-specific amplicons in PCR assays.

We used whole genome sequencing to... Read More about Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae (2018)
Journal Article
Antenucci, F., Fougeroux, C., Deeney, A., Orskov, C., Rycroft, A. N., Holst, P. J., & Bojesen, A. M. (2018). In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae. Veterinary Research, 49, 4. https://doi.org/10.1186/s13567-017-0502-x

Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the... Read More about In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae.

Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs (2018)
Journal Article
Brockmeier, S. L., Loving, C. L., Nicholson, T. L., Wang, J., Peters, S. E., Weinert, L., Chaudhuri, R., Seilly, D. J., Langford, P. R., Rycroft, A. N., Wren, B. W., Maskell, D. J., & Tucker, A. W. (2018). Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs. Infection and Immunity, 86(3), e00559. https://doi.org/10.1128/IAI.00559-17

Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes... Read More about Use of proteins identified through a functional genomic screen to develop a protein subunit vaccine that provides significant protection against virulent Streptococcus suis in pigs.

Zoonotic potential of Salmonella enterica carried by pet tortoises (2017)
Journal Article
Bruce, H. L., Barrow, P. A., & Rycroft, A. N. (2017). Zoonotic potential of Salmonella enterica carried by pet tortoises. Veterinary Record, 182(5), 141. https://doi.org/10.1136/vr.104457

The prevalence of Salmonella in chelonians is not known in the UK and it is not clear whether such Salmonella strains would be pathogenic for human beings. Some strains, such as members of the Arizonae subgroup, may be unable to cause anything more t... Read More about Zoonotic potential of Salmonella enterica carried by pet tortoises.

Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014 (2017)
Journal Article
Hernandez-Garcia, J., Wang, J., Restif, O., Holmes, M. A., Mather, A. E., Weinert, L. A., Wileman, T. M., Thomson, J. R., Langford, P. R., Wren, B. W., Rycroft, A. N., Maskell, D. J., & Tucker, A. W. (2017). Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014. Veterinary Microbiology, 207, 117-124. https://doi.org/10.1016/j.vetmic.2017.06.002

Antimicrobial resistance in Streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time.

We compared patterns of resistance between S. suis isolat... Read More about Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.

Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae (2017)
Journal Article
Bossé, J. T., Li, Y., Rogers, J., Fernandez Crespo, R., Li, Y., Chaudhuri, R. R., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., & Langford, P. R. (2017). Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 8(311), https://doi.org/10.3389/fmicb.2017.00311

The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole... Read More about Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae.

The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering (2017)
Journal Article
Cuccui, J., Terra, V. S., Bossé, J. T., Naegeli, A., Abouelhadid, S., Li, Y. W., Lin, C. W., Vohra, P., Tucker, A. W., Rycroft, A. N., Maskell, D. J., Aebi, M., Langford, P. R., & Wren, B. W. (2017). The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering. Open Biology, 7(1), https://doi.org/10.1098/rsob.160212

A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay (2017)
Journal Article
Bossé, J. T., Li, Y., Sárközi, R., Gottschalk, M., Angen, Ø., Nedbalcova, K., Rycroft, A. N., Fodor, L., & Langford, P. R. (2017). A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay. Journal of Clinical Microbiology, 55(3), 902-907. https://doi.org/10.1128/JCM.02166-16

Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a p... Read More about A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.

ICEApl1, an Integrative Conjugative Element Related to ICEHin1056, Identified in the Pig Pathogen Actinobacillus pleuropneumoniae (2016)
Journal Article
Bossé, J. T., Li, Y., Fernandez Crespo, R., Chaudhuri, R. R., Rogers, J., Holden, M. T., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., & BRaDP1T Consortium. (2016). ICEApl1, an Integrative Conjugative Element Related to ICEHin1056, Identified in the Pig Pathogen Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 7, https://doi.org/10.3389/fmicb.2016.00810

Whole genome investigation of a divergent clade of the pathogen Streptococcus suis (2015)
Journal Article
Baig, A., Weinert, L. A., Peters, S. E., Howell, K. J., Chaudhuri, R. R., Wang, J., Holden, M. T. G., Parkhill, J., Langford, P. R., Rycroft, A. N., Wren, B. W., Tucker, A. W., Maskell, D. J., & BRaDP1T Consortium. (2015). Whole genome investigation of a divergent clade of the pathogen Streptococcus suis. Frontiers in Microbiology, 6(1), https://doi.org/10.3389/fmicb.2015.01191

Streptococcus suis is a major porcine and zoonotic pathogen responsible for significant economic losses in the pig industry and an increasing number of human cases. Multiple isolates of S. suis show marked genomic diversity. Here, we report the analy... Read More about Whole genome investigation of a divergent clade of the pathogen Streptococcus suis.

Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae (2015)
Journal Article
Bossé, J. T., Li, Y., Walker, S., Atherton, T., Crespo, R. F., Williamson, S. M., Rogers, J., Chaudhuri, R. R., Weinert, L. A., Oshota, O., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., & BRaDP1T Consortium. (2015). Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae. Journal of Antimicrobial Chemotherapy, 70(8), 2217-2222. https://doi.org/10.1093/jac/dkv121

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation (2014)
Journal Article
Bossé, J. T., Soares-Bazzolli, D. M., Li, Y., Wren, B. W., Tucker, A. W., Maskell, D. J., Rycroft, A. N., Langford, P. R., & BRaDP1T Consortium. (2014). The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation. PLoS ONE, 9(11), https://doi.org/10.1371/journal.pone.0111252

We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinase... Read More about The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation.