Pyrophosphate: a key inhibitor of mineralisation
(2016)
Journal Article
Orriss, I. R., Arnett, T. R., & Russell, R. G. G. (2016). Pyrophosphate: a key inhibitor of mineralisation. Current Opinion in Pharmacology, 28, 57-68. https://doi.org/10.1016/j.coph.2016.03.003
All Outputs (47)
Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro (2016)
Journal Article
Hajjawi, M. O. R., Patel, J. J., Corcelli, M., Arnett, T. R., & Orriss, I. R. (2016). Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro. Purinergic Signalling, 12(2), 247-258. https://doi.org/10.1007/s11302-016-9499-2Extracellular ATP, signalling through P2 receptors, exerts well-documented effects on bone cells, inhibiting mineral deposition by osteoblasts and stimulating the formation and resorptive activity of osteoclasts. The aims of this study were to determ... Read More about Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro.
Chronic administration of Glucagon-like peptide-1 receptor agonists improves trabecular bone mass and architecture in ovariectomised mice (2015)
Journal Article
Pereira, M., Jeyabalan, J., Jorgensen, C. S., Hopkinson, M., Al-Jazzar, A., Roux, J. P., Chavassieux, P., Orriss, I. R., Cleasby, M. E., & Chenu, C. (2015). Chronic administration of Glucagon-like peptide-1 receptor agonists improves trabecular bone mass and architecture in ovariectomised mice. BONE, 81, 459-67. https://doi.org/10.1016/j.bone.2015.08.006Some anti-diabetic therapies can have adverse effects on bone health and increase fracture risk. In this study, we tested the skeletal effects of chronic administration of two Glucagon-like peptide-1 receptor agonists (GLP-1RA), increasingly used for... Read More about Chronic administration of Glucagon-like peptide-1 receptor agonists improves trabecular bone mass and architecture in ovariectomised mice.
Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity (2015)
Journal Article
Orriss, I. R., Key, M. L., Hajjawi, M. O. R., Millan, J. L., & Arnett, T. R. (2015). Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity. Journal of Cellular Physiology, 230(12), 3049-3056. https://doi.org/10.1002/jcp.25041Previous work has shown that acidosis prevents bone nodule formation by osteoblasts in vitro by inhibiting mineralisation of the collagenous matrix. The ratio of phosphate (Pi) to pyrophosphate (PPi) in the bone microenvironment is a fundamental regu... Read More about Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity.
Mineralisation of collagen rich soft tissues and osteocyte lacunae in Enpp1-/- mice (2014)
Journal Article
Hajjawi, M. O. R., MacRae, V. E., Huesa, C., Boyde, A., Millan, J. L., Arnett, T. R., & Orriss, I. R. (2014). Mineralisation of collagen rich soft tissues and osteocyte lacunae in Enpp1-/- mice. BONE, 69, 139-147. https://doi.org/10.1016/j.bone.2014.09.016Ecto-nucleotide pyrophosphatase/phosphodiesterases (NPPs) hydrolyse nucleotide triphosphates to the corresponding nucleotide monophosphates and the mineralisation inhibitor, pyrophosphate (PPi). This study examined the role of NPP1 in osteocytes, ost... Read More about Mineralisation of collagen rich soft tissues and osteocyte lacunae in Enpp1-/- mice.
FIBRILLIN-1 EXPRESSION, WHICH REGULATES OF TGF-B BIOAVAILABILITY, IS MODIFIED DURING OSTEOARTHRITIS AND MUTATIONS LEAD TO OSTEOARTHRITIS
Journal Article
Ramanayake, W., Jones, H., Orriss, I. R., Arnett, T., Pitsillides, A. A., Denton, C., Abraham, D., & Poulet, B. FIBRILLIN-1 EXPRESSION, WHICH REGULATES OF TGF-B BIOAVAILABILITY, IS MODIFIED DURING OSTEOARTHRITIS AND MUTATIONS LEAD TO OSTEOARTHRITIS
Hypoxia stimulates vesicular ATP release from rat osteoblasts
Journal Article
Orriss, I. R., Knight, G. E., Utting, J. C., Taylor, S. E., Burnstock, G., & Arnett, T. R. Hypoxia stimulates vesicular ATP release from rat osteoblasts. Journal of Cellular Physiology, 220(1), 155-62. https://doi.org/10.1002/jcp.21745
Inhibition of osteoblast function in vitro by aminobisphosphonates
Journal Article
Orriss, I. R., Key, M. L., Colston, K. W., & Arnett, T. R. Inhibition of osteoblast function in vitro by aminobisphosphonates. Journal of Cellular Biochemistry, 106(1), 109-18. https://doi.org/10.1002/jcb.21983
The P2Y(6) receptor stimulates bone resorption by osteoclasts
Journal Article
Orriss, I. R., Wang, N., Burnstock, G., Arnett, T. R., Gartland, A., Robaye, B., & Boeynaems, J. M. The P2Y(6) receptor stimulates bone resorption by osteoclasts. Endocrinology, 152(10), 3706-16. https://doi.org/10.1210/en.2011-1073
Clopidogrel (Plavix), a P2Y12 receptor antagonist, inhibits bone cell function in vitro and decreases trabecular bone in vivo
Journal Article
Syberg, S., Brandao-Burch, A., Patel, J. J., Hajjawi, M., Arnett, T. R., Schwarz, P., Jorgensen, N. R., & Orriss, I. R. Clopidogrel (Plavix), a P2Y12 receptor antagonist, inhibits bone cell function in vitro and decreases trabecular bone in vivo. Journal of Bone and Mineral Research, 27(11), 2373-86. https://doi.org/10.1002/jbmr.1690
Rat osteoblast cultures
Journal Article
Orriss, I. R., Taylor, S. E., & Arnett, T. R. Rat osteoblast cultures. https://doi.org/10.1007/978-1-61779-415-5_3
The regulation of osteoblast function and bone mineralisation by extracellular nucleotides: The role of p2x receptors
Journal Article
Orriss, I. R., Key, M. L., Brandao-Burch, A., Patel, J. J., Burnstock, G., & Arnett, T. R. The regulation of osteoblast function and bone mineralisation by extracellular nucleotides: The role of p2x receptors. BONE, 51(3), 389-400. https://doi.org/10.1016/j.bone.2012.06.013
Rodent osteoclast cultures
Journal Article
Orriss, I. R., & Arnett, T. R. Rodent osteoclast cultures. https://doi.org/10.1007/978-1-61779-415-5_8
The P2X7 receptor is an important regulator of extracellular ATP levels
Journal Article
Brandao-Burch, A., Key, M. L., Patel, J. J., Arnett, T. R., & Orriss, I. R. The P2X7 receptor is an important regulator of extracellular ATP levels. Frontiers in Endocrinology, 3(41), https://doi.org/10.3389/fendo.2012.00041
Role of the P2Y13 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes
Journal Article
Biver, G., Wang, N., Gartland, A., Orriss, I. R., Arnett, T. R., Boeynaems, J. M., & Robaye, B. Role of the P2Y13 receptor in the differentiation of bone marrow stromal cells into osteoblasts and adipocytes. STEM CELLS, https://doi.org/10.1002/stem.1411
Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation
Journal Article
Orriss, I. R., Key, M. L., Hajjawi, M. O. R., & Arnett, T. R. (in press). Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation. PLoS ONE, 8(7), e69057. https://doi.org/10.1371/journal.pone.0069057Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent mech... Read More about Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation.
Generation of rodent and human osteoblasts
Journal Article
Taylor, S. E., Shah, M., & Orriss, I. R. Generation of rodent and human osteoblasts. https://doi.org/10.1038/bonekey.2014.80
THE REGULATION OF SKELETAL AND SOFT TISSUE MINERALIZATION BY ATP AND NPP1
Presentation / Conference Contribution
Orriss, I. R., Hajjawi, M. O. R., & Arnett, T. R. THE REGULATION OF SKELETAL AND SOFT TISSUE MINERALIZATION BY ATP AND NPP1
Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures
Journal Article
Wornham, D. P., Hajjawi, M. O. R., Orriss, I. R., & Arnett, T. R. (in press). Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures. Osteoporosis International, 25(10), 2477-2484. https://doi.org/10.1007/s00198-014-2791-5The basic mechanisms by which strontium ranelate acts on bone are still unclear. We show that an important action of strontium salts is to block calcification in cultures of osteoblasts, the bone-forming cells. These results suggest that strontium tr... Read More about Strontium potently inhibits mineralisation in bone-forming primary rat osteoblast cultures and reduces numbers of osteoclasts in mouse marrow cultures.
Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats
Journal Article
Orriss, I. R., Hajjawi, M. O. R., Huesa, C., MacRae, V. E., & Arnett, T. R. (in press). Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats. International Journal of Molecular Medicine, 34(5), 1201-1208. https://doi.org/10.3892/ijmm.2014.1926The in vitro culture of calvarial osteoblasts from neonatal rodents remains an important method for studying the regulation of bone formation. The widespread use of transgenic mice has created a particular need for a reliable, simple method that allo... Read More about Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats.