Skip to main content

Research Repository

Advanced Search

Outputs (28)

Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria (2022)
Journal Article
Gibson, A. J., Stiens, J., Passmore, I. J., Faulkner, V., Miculob, J., Willcocks, S., Coad, M., Berg, S., Werling, D., Wren, B. W., Nobeli, I., Villarreal-Ramos, B., & Kendall, S. L. (2022). Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria. mBio, https://doi.org/10.1128/mbio.00672-22

Tuberculosis has severe impacts in both humans and animals. Understanding the genetic basis of survival of both Mycobacterium tuberculosis, the human adapted species, and Mycobacterium bovis, the animal adapted species is crucial to deciphering the b... Read More about Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria.

Probing differences in gene essentiality between the human and animal adapted (2021)
Journal Article
Gibson, A. J., Passmore, I. J., Faulkner, V., Xia, D., Nobeli, I., Stiens, J., Clarke, T. G., Sobkowiak, B., Willcocks, S., Werling, D., Villarreal-Ramos, B., Wren, B. W., & Kendall, S. L. (2021). Probing differences in gene essentiality between the human and animal adapted. Frontiers in Veterinary Science,

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, ho... Read More about Probing differences in gene essentiality between the human and animal adapted.

CD23 is a glycan-binding receptor in some mammalian species (2019)
Journal Article
Jégouzo, S. A. F., Feinberg, H., Morrison, A., Holder, A., May, A., Huang, Z., Jiang, L., Lasanajak, Y., Smith, D. F., Werling, D., Drickamer, K., Weis, W. I., & Taylor, M. E. (2019). CD23 is a glycan-binding receptor in some mammalian species. Journal of Biological Chemistry, https://doi.org/10.1074/jbc.RA119.010572

CD23, the low affinity IgE receptor found on B lymphocytes and other cells, contains a C-terminal lectin-like domain that resembles C-type carbohydrate-recognition domains (CRDs) found in many glycan-binding receptors. In most mammalian species, the... Read More about CD23 is a glycan-binding receptor in some mammalian species.

Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows (2019)
Journal Article
Tombacz, K., Peters, L. M., Chang, Y. M., Steele, M., Werling, D., & Gibson, A. J. (2019). Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows. Journal of Dairy Science, 102(10), 9389-9395. https://doi.org/10.3168/jds.2019-16563

In periparturient dairy cows, immune suppression, resulting in decreased neutrophil numbers and function, leads to increased susceptibility to postpartum conditions such as mastitis, retained placenta, and metritis. Administration of polyethylene gly... Read More about Pegbovigrastim treatment in vivo does not affect granulocyte ability to migrate to endometrial cells and kill bacteria in vitro in healthy cows.

Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence (2019)
Journal Article
García-Sánchez, M., Jiménez-Pelayo, L., Horcajo, P., Regidor-Cerrillo, J., Ólafsson, E. B., Bhandage, A. K., Barragan, A., Werling, D., Ortega-Mora, L. M., & Collantes-Fernández, E. (2019). Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence. Frontiers in Immunology, 10(915), https://doi.org/10.3389/fimmu.2019.00915

Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, represents one of the main causes of abortion in cattle. Macrophages (MØs) are mediators of the innate immune response against infection and likely one of the first cells en... Read More about Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence.

Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5 (2018)
Journal Article
Tombacz, K., Burgess, G., Holder, A. L., Werners, A., & Werling, D. (2018). Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5. Innate Immunity, https://doi.org/10.1177/1753425918798450

Toxoplasma gondii is responsible for one of the most prevalent infections in people. T. gondii profilin (TgPr) is a protein integral to parasite movement and cellular invasion. Murine TLR has been described to bind TgPr. Furthermore, more recently, h... Read More about Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5.

Genome-wide association studies of inflammatory bowel disease in German shepherd dogs (2018)
Journal Article
Perivan, A., Bertolini, F., Rothschild, M. F., Simpson, K. W., Jergens, A. E., Allenspach, K., & Werling, D. (2018). Genome-wide association studies of inflammatory bowel disease in German shepherd dogs. PLoS ONE, 13(7), e0200685. https://doi.org/10.1371/journal.pone.0200685

Canine Inflammatory Bowel Disease (IBD) is considered a multifactorial disease caused by complex interactions between the intestinal immune system, intestinal microbiota and environmental factors in genetically susceptible individuals. Although IBD c... Read More about Genome-wide association studies of inflammatory bowel disease in German shepherd dogs.

A Prospective, Randomized, Blinded, Placebo-Controlled Pilot Study on the Effect of Enterococcus faecium on Clinical Activity and Intestinal Gene Expression in Canine Food-Responsive Chronic Enteropathy (2015)
Journal Article
Schmitz, S., Glanemann, B., Garden, O. A., Brooks, H. W., Chang, Y. M., Werling, D., & Allenspach, K. (2015). A Prospective, Randomized, Blinded, Placebo-Controlled Pilot Study on the Effect of Enterococcus faecium on Clinical Activity and Intestinal Gene Expression in Canine Food-Responsive Chronic Enteropathy. Journal of Veterinary Internal Medicine, 29(2), 533-43. https://doi.org/10.1111/jvim.12563

Two TIR-like domain containing proteins in a newly emerging zoonotic Staphylococcus aureus strain sequence type 398 are potential virulence factors by impacting on the host innate immune response (2014)
Journal Article
Patterson, N. J., Günther, J., Gibson, A. J., Offord, V., Coffey, T. J., Splitter, G., Monk, I., Seyfert, H. M., & Werling, D. (2014). Two TIR-like domain containing proteins in a newly emerging zoonotic Staphylococcus aureus strain sequence type 398 are potential virulence factors by impacting on the host innate immune response. Frontiers in Microbiology, 5(662), https://doi.org/10.3389/fmicb.2014.00662

Staphylococcus aureus, sequence type (ST) 398, is an emerging pathogen and the leading cause of livestock-associated methicillin-resistant S. aureus infections in Europe and North America. This strain is characterized by high promiscuity in terms of... Read More about Two TIR-like domain containing proteins in a newly emerging zoonotic Staphylococcus aureus strain sequence type 398 are potential virulence factors by impacting on the host innate immune response.

Global Gene Expression Profiling of Myeloid Immune Cell Subsets in Response to In Vitro Challenge with Porcine Circovirus 2b (2014)
Journal Article
Mavrommatis, B., Offord, V., Patterson, R., Watson, M., Kanellos, T., Steinbach, F., Grierson, S., & Werling, D. (2014). Global Gene Expression Profiling of Myeloid Immune Cell Subsets in Response to In Vitro Challenge with Porcine Circovirus 2b. PLoS ONE, 9(3), e91081. https://doi.org/10.1371/journal.pone.0091081

Compelling evidence suggests that the early interaction between porcine circovirus 2 (PCV-2) and the innate immune system is the key event in the pathogenesis of Post-Weaning Multisystemic Wasting Syndrome (PMWS). Furthermore, PCV2 has been detected... Read More about Global Gene Expression Profiling of Myeloid Immune Cell Subsets in Response to In Vitro Challenge with Porcine Circovirus 2b.

Stimulation of Duodenal Biopsies and Whole Blood from Dogs with Food-Responsive Chronic Enteropathy and Healthy Dogs with Toll-Like Receptor Ligands and Probiotic Enterococcus faecium
Journal Article
Schmitz, S., Henrich, M., Neiger, R., Werling, D., & Allenspach, K. Stimulation of Duodenal Biopsies and Whole Blood from Dogs with Food-Responsive Chronic Enteropathy and Healthy Dogs with Toll-Like Receptor Ligands and Probiotic Enterococcus faecium. Scandinavian Journal of Immunology, https://doi.org/10.1111/sji.12186

Lack of Genetic Diversity in Newly Sequenced Porcine Circovirus Type 1 Strains Isolated 20 Years Apart
Journal Article
Tombacz, K., Patterson, R., Grierson, S. S., & Werling, D. (in press). Lack of Genetic Diversity in Newly Sequenced Porcine Circovirus Type 1 Strains Isolated 20 Years Apart. Genome Announcements, 2(2), e00156-14. https://doi.org/10.1128/genomeA.00156-14

The complete genome sequences of a porcine circovirus type 1 (PCV1) strain isolated in 1990 and one isolated in 2011 were obtained and compared to the sequences of other available PCV1 isolates. Phylogenetic analyses revealed very low genetic diversi... Read More about Lack of Genetic Diversity in Newly Sequenced Porcine Circovirus Type 1 Strains Isolated 20 Years Apart.

The Phylogeography of Rabies in Grenada, West Indies, and Implications for Control
Journal Article
Zieger, U., Marston, D. A., Sharma, R., Chikweto, A., Tiwari, K., Sayyid, M., Louison, B., Goharriz, H., Voller, K., Breed, A. C., Werling, D., Fooks, A. R., & Horton, D. L. (in press). The Phylogeography of Rabies in Grenada, West Indies, and Implications for Control. PLoS Neglected Tropical Diseases, 8(10), e3251. https://doi.org/10.1371/journal.pntd.0003251

In Grenada, West Indies, rabies is endemic, and is thought to be maintained in a wildlife host, the small Indian mongoose (Herpestes auropunctatus) with occasional spillover into other hosts. Therefore, the present study was undertaken to improve und... Read More about The Phylogeography of Rabies in Grenada, West Indies, and Implications for Control.