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The analysis of acetaminophen (paracetamol) and seven metabolites in rat, pig and human plasma by U(H)PLC–MS

Dargue, R; Grant, I; Nye, L C; Nicholls, A; Dare, T; Stahl, S H; Plumb, R S; Lee, K C L

Authors

R Dargue

I Grant

L C Nye

A Nicholls

T Dare

S H Stahl

R S Plumb

K C L Lee



Abstract

A U(H)PLC–MS/MS method is described for the analysis of acetaminophen and its sulphate, glucuronide, glutathione, cysteinyl and N-acetylcysteinyl metabolites in plasma using stable isotope-labeled internal standards. P-Aminophenol glucuronide and 3-methoxyacetaminophen were monitored and semi-quantified using external standards. The assay takes 7.5 min/sample, requires only 5 μl of plasma and involves minimal sample preparation. The method was validated for rat plasma and cross validated for human and pig plasma and mouse serum. LOQ in plasma for these analytes were 0.44 μg/ml (APAP-C), 0.58 μg/ml (APAP-SG), 0.84 μg/ml (APAP-NAC), 2.75 μg/ml (APAP-S), 3.00 μg/ml (APAP-G) and 16 μg/ml (APAP). Application of the method is illustrated by the analysis of plasma following oral administration of APAP to male Han Wistar rats.

Citation

Dargue, R., Grant, I., Nye, L. C., Nicholls, A., Dare, T., Stahl, S. H., …Lee, K. C. L. (2020). The analysis of acetaminophen (paracetamol) and seven metabolites in rat, pig and human plasma by U(H)PLC–MS. Bioanalysis, 12(7), https://doi.org/10.4155/bio-2020-0015

Journal Article Type Article
Acceptance Date Mar 23, 2020
Publication Date Apr 28, 2020
Deposit Date May 11, 2020
Publicly Available Date Mar 28, 2024
Journal Bioanalysis
Print ISSN 1757-6180
Publisher Newlands Press
Peer Reviewed Peer Reviewed
Volume 12
Issue 7
DOI https://doi.org/10.4155/bio-2020-0015
Public URL https://rvc-repository.worktribe.com/output/1377593

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