Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity

Gohin, S; Javaheri, B; Hopkinson, M; Pitsillides, A A; Arnett, T R; Chenu, Chantal

Abstract

Blood supply is essential for osteogenesis, yet its relationship to load-related increases in bone mass is poorly defined. Herein, we aim to investigate the link between load-induced osteogenesis and the blood supply (bone perfusion and vascular porosity) using an established osteogenic non-invasive model of axial loading. Accordingly, 12N mechanical loads were applied to the right tibiae of six male C57BL6 mice at 10-12 weeks of age, 3 times/week for two weeks. Skeletal perfusion was measured acutely (post-loading) and chronically in loaded and contralateral, non-loaded hindlimbs by Laser Doppler imaging. Vascular and lacunar porosity of the cortical bone and tibia load-related changes in trabecular and cortical bone were measured by nanoCT and micro-CT, respectively. We found that the mean skeletal perfusion (loaded: non-loaded limb ratio) increased by 56% immediately following the first loading episode (versus baseline, P

Citation

Gohin, S., Javaheri, B., Hopkinson, M., Pitsillides, A. A., Arnett, T. R., & Chenu, C. (2020). Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity. Journal of Applied Physiology,

• Journal Article Type: Article
• Acceptance Date: Mar 6, 2020
• Publication Date: Mar 12, 2020
• Deposit Date: Mar 24, 2020
• Journal: Journal of Applied Physiology
• Print ISSN: 8750-7587
• Publisher: American Physiological Society
• Peer Reviewed: Not Peer Reviewed
• Public URL: https://rvc-repository.worktribe.com/output/1378225
• Publisher URL: https://doi.org/10.1152/japplphysiol.00416.2019