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Evaluation of the Immunoprotective Potential of Recombinant Paraflagellar Rod Proteins of Trypanosoma evansi in Mice

Maharana, B R; Sudhakar, N R; Jawalagatti, V; Saravanan, B C; Blake, D P; Tewari, A K

Authors

B R Maharana

N R Sudhakar

V Jawalagatti

B C Saravanan

D P Blake

A K Tewari



Abstract

Trypanosomosis, caused by Trypanosoma evansi, is an economically significant disease of livestock. Systematic antigenic variation by the parasite has undermined prospects for the development of a protective vaccine that targets the immunodominant surface antigens, encouraging exploration of alternatives. The paraflagellar rod (PFR), constituent proteins of the flagellum, are prominent non-variable vaccine candidates for T. evansi owing to their strategic location. Two major PFR constituent proteins, PFR1 (1770bp) and PFR2 (1800bp), were expressed using Escherichia coli. Swiss albino mice were immunized with the purified recombinant TePFR1 (89KDa) and TePFR2 (88KDa) proteins, as well as with the mix of the combined proteins at equimolar concentrations, and subsequently challenged with virulent T. evansi. The PFR-specific humoral response was assessed by ELISA. Cytometric bead-based assay was used to measure the cytokine response and flow cytometry for quantification of the cytokines. The recombinant TePFR proteins induced specific humoral responses in mice, including IgG1 followed by IgG2a and IgG2b. A balanced cytokine response induced by rTePFR 1 and 2 protein vaccination associated with extended survival and improved control of parasitemia following lethal challenge. The observation confirms the immunoprophylactic potential of the covert antigens of T. evansi.

Citation

Maharana, B. R., Sudhakar, N. R., Jawalagatti, V., Saravanan, B. C., Blake, D. P., & Tewari, A. K. (2020). Evaluation of the Immunoprotective Potential of Recombinant Paraflagellar Rod Proteins of Trypanosoma evansi in Mice. Vaccines, 8(1), 84. https://doi.org/10.3390/vaccines8010084

Journal Article Type Article
Acceptance Date Jan 27, 2020
Publication Date Feb 12, 2020
Deposit Date Feb 18, 2020
Publicly Available Date Mar 28, 2024
Journal Vaccines
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Pages 84
DOI https://doi.org/10.3390/vaccines8010084
Public URL https://rvc-repository.worktribe.com/output/1378670

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