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Transcriptional profiling unveils type I and II interferon networks in blood and tissues across diseases

Singhania, A; Graham, C M; Gabryšová, L; Moreira-Teixeira, L; Stavropoulos, E; Pitt, J M; Chakravarty, P; Warnatsch, A; Branchett, W J; Conejero, L; Lin, J-W; Davidson, S; Wilson, M S; Bancroft, G; Langhorne, J; Frickel, E; Sesay, A K; Priestnall, S L; Herbert, E; Ioannou, M; Wang, Q; Humphreys, I R; Dodd, J; Openshaw, P J M; Mayer-Barber, K D; Jankovic, D; Sher, A; Lloyd, C M; Baldwin, N; Chaussabel, D; Papayannopoulos, V; Wack, A; Banchereau, J F; Pascual, V M; O’Garra, A


A Singhania

C M Graham

L Gabryšová

L Moreira-Teixeira

E Stavropoulos

J M Pitt

P Chakravarty

A Warnatsch

W J Branchett

L Conejero

J-W Lin

S Davidson

M S Wilson

G Bancroft

J Langhorne

E Frickel

A K Sesay

S L Priestnall

E Herbert

M Ioannou

Q Wang

I R Humphreys

J Dodd

P J M Openshaw

K D Mayer-Barber

D Jankovic

A Sher

C M Lloyd

N Baldwin

D Chaussabel

V Papayannopoulos

A Wack

J F Banchereau

V M Pascual

A O’Garra


Understanding how immune challenges elicit different responses is critical for diagnosing and deciphering immune regulation. Using a modular strategy to interpret the complex transcriptional host response in mouse models of infection and inflammation, we show a breadth of immune responses in the lung. Lung immune signatures are dominated by either IFN-γ and IFN-inducible, IL-17-induced neutrophil- or allergy-associated gene expression. Type I IFN and IFN-γ-inducible, but not IL-17- or allergy-associated signatures, are preserved in the blood. While IL-17-associated genes identified in lung are detected in blood, the allergy signature is only detectable in blood CD4+ effector cells. Type I IFN-inducible genes are abrogated in the absence of IFN-γ signaling and decrease in the absence of IFNAR signaling, both independently contributing to the regulation of granulocyte responses and pathology during Toxoplasma gondii infection. Our framework provides an ideal tool for comparative analyses of transcriptional signatures contributing to protection or pathogenesis in disease.


Singhania, A., Graham, C. M., Gabryšová, L., Moreira-Teixeira, L., Stavropoulos, E., Pitt, J. M., …O’Garra, A. (2019). Transcriptional profiling unveils type I and II interferon networks in blood and tissues across diseases. Nature Communications, 10,

Journal Article Type Article
Acceptance Date May 9, 2019
Publication Date Jun 28, 2019
Deposit Date Aug 2, 2019
Publicly Available Date Aug 5, 2019
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 10
Public URL


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