E Knuepfer
Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites
Knuepfer, E; Wright, K E; Prajapati, S K; Rawlinson, T A; Mohring, F; Koch, M; Lyth, O R; Howell, S A; Villasis, E; Snijders, A P; Moon, R W; Draper, S J; Rosanas-Urgell, A; Higgins, M K; Baum, J; Holder, A A
Authors
K E Wright
S K Prajapati
T A Rawlinson
F Mohring
M Koch
O R Lyth
S A Howell
E Villasis
A P Snijders
R W Moon
S J Draper
A Rosanas-Urgell
M K Higgins
J Baum
A A Holder
Abstract
Malaria is caused by Plasmodium parasites, which invade and replicate in erythrocytes. For Plasmodium falciparum, the major cause of severe malaria in humans, a heterotrimeric complex comprised of the secreted parasite proteins, PfCyRPA, PfRIPR and PfRH5 is essential for erythrocyte invasion, mediated by the interaction between PfRH5 and erythrocyte receptor basigin (BSG). However, whilst CyRPA and RIPR are present in most Plasmodium species, RH5 is found only in the small Laverania subgenus. Existence of a complex analogous to PfRH5-PfCyRPA-PfRIPR targeting BSG, and involvement of CyRPA and RIPR in invasion, however, has not been addressed in non-Laverania parasites. Here, we establish that unlike P. falciparum, P. knowlesi and P. vivax do not universally require BSG as a host cell invasion receptor. Although we show that both PkCyRPA and PkRIPR are essential for successful invasion of erythrocytes by P. knowlesi parasites in vitro, neither protein forms a complex with each other or with an RH5-like molecule. Instead, PkRIPR is part of a different trimeric protein complex whereas PkCyRPA appears to function without other parasite binding partners. It therefore appears that in the absence of RH5, outside of the Laverania subgenus, RIPR and CyRPA have different, independent functions crucial for parasite survival.
Citation
Knuepfer, E., Wright, K. E., Prajapati, S. K., Rawlinson, T. A., Mohring, F., Koch, M., Lyth, O. R., Howell, S. A., Villasis, E., Snijders, A. P., Moon, R. W., Draper, S. J., Rosanas-Urgell, A., Higgins, M. K., Baum, J., & Holder, A. A. (2019). Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites. PLoS Pathogens, 15(6), e1007809. https://doi.org/10.1371/journal.ppat.1007809
Journal Article Type | Article |
---|---|
Acceptance Date | May 1, 2019 |
Publication Date | Jun 11, 2019 |
Deposit Date | Jan 23, 2020 |
Publicly Available Date | Jan 27, 2020 |
Journal | PLoS Pathogens |
Print ISSN | 1553-7366 |
Electronic ISSN | 1553-7374 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 15 |
Issue | 6 |
Pages | e1007809 |
DOI | https://doi.org/10.1371/journal.ppat.1007809 |
Public URL | https://rvc-repository.worktribe.com/output/1381839 |
Files
12532_Divergent-roles-for-the-RH5-complex-components-CyRPA-and-RIPR-in-human-infective-malaria-parasites.pdf
(3.4 Mb)
PDF
You might also like
The PfRCR complex bridges malaria parasite and erythrocyte during invasion
(2023)
Journal Article
Downloadable Citations
About RVC Repository
Administrator e-mail: publicationsrepos@rvc.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search