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Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites

Knuepfer, E; Wright, K E; Prajapati, S K; Rawlinson, T A; Mohring, F; Koch, M; Lyth, O R; Howell, S A; Villasis, E; Snijders, A P; Moon, R W; Draper, S J; Rosanas-Urgell, A; Higgins, M K; Baum, J; Holder, A A


E Knuepfer

K E Wright

S K Prajapati

T A Rawlinson

F Mohring

M Koch

O R Lyth

S A Howell

E Villasis

A P Snijders

R W Moon

S J Draper

A Rosanas-Urgell

M K Higgins

J Baum

A A Holder


Malaria is caused by Plasmodium parasites, which invade and replicate in erythrocytes. For Plasmodium falciparum, the major cause of severe malaria in humans, a heterotrimeric complex comprised of the secreted parasite proteins, PfCyRPA, PfRIPR and PfRH5 is essential for erythrocyte invasion, mediated by the interaction between PfRH5 and erythrocyte receptor basigin (BSG). However, whilst CyRPA and RIPR are present in most Plasmodium species, RH5 is found only in the small Laverania subgenus. Existence of a complex analogous to PfRH5-PfCyRPA-PfRIPR targeting BSG, and involvement of CyRPA and RIPR in invasion, however, has not been addressed in non-Laverania parasites. Here, we establish that unlike P. falciparum, P. knowlesi and P. vivax do not universally require BSG as a host cell invasion receptor. Although we show that both PkCyRPA and PkRIPR are essential for successful invasion of erythrocytes by P. knowlesi parasites in vitro, neither protein forms a complex with each other or with an RH5-like molecule. Instead, PkRIPR is part of a different trimeric protein complex whereas PkCyRPA appears to function without other parasite binding partners. It therefore appears that in the absence of RH5, outside of the Laverania subgenus, RIPR and CyRPA have different, independent functions crucial for parasite survival.


Knuepfer, E., Wright, K. E., Prajapati, S. K., Rawlinson, T. A., Mohring, F., Koch, M., …Holder, A. A. (2019). Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites. PLoS Pathogens, 15(6), e1007809.

Journal Article Type Article
Acceptance Date May 1, 2019
Publication Date Jun 11, 2019
Deposit Date Jan 23, 2020
Publicly Available Date Jan 27, 2020
Journal PLoS Pathogens
Print ISSN 1553-7366
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 15
Issue 6
Pages e1007809
Public URL


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