A Mead
Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media
Mead, A; Lees, P; Mitchell, J; Rycroft, A N; Standing, J F; Toutain, P L; Pelligand, L
Authors
P Lees
J Mitchell
A N Rycroft
J F Standing
P L Toutain
L Pelligand
Abstract
For clinical isolates of bovine Mannheimia haemolytica and Pasteurella multocida, this study reports minimum inhibitory concentration (MIC) differences for tetracycline, oxytetracycline and doxycycline between cation‐adjusted Mueller‐Hinton broth (CAMHB), foetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) medium. MICs were determined according to CLSI standards and additionally using five overlapping sets of twofold dilutions. Matrix effect: (a) free drug MICs and minimum bactericidal concentrations (MBC) for all drugs were significantly higher in FBS than in CAMHB for both pathogens (p < 0.001); (b) MICs and MBCs were higher for CAMHB and FBS compared to RPMI for P. multocida only. Net growth rate for P. multocida in CAMHB was significantly slower than in FBS and higher than in RPMI, correlating to MIC and MBC ranking. Drug effect: doxycycline MICs and MBCs were significantly lower (p < 0.001) in both CAMHB and FBS than tetracycline and oxytetracycline for both pathogens. Only for M. haemolytica were oxytetracycline MIC and MBC significantly lower than tetracycline, precluding the use of tetracycline to predict oxytetracycline susceptibility in this species. Determining potencies of tetracyclines in a physiological medium, such as FBS, is proposed, when the objective is correlation with pharmacokinetic data for dosage determination.
Citation
Mead, A., Lees, P., Mitchell, J., Rycroft, A. N., Standing, J. F., Toutain, P. L., & Pelligand, L. (2019). Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media. Journal of Veterinary Pharmacology and Therapeutics, 42(1), 52-59. https://doi.org/10.1111/jvp.12719
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 1, 2018 |
Publication Date | Jan 1, 2019 |
Deposit Date | Oct 6, 2018 |
Publicly Available Date | Mar 29, 2024 |
Journal | JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS |
Print ISSN | 0140-7783 |
Publisher | American Academy of Veterinary Pharmacology and Therapeutics |
Peer Reviewed | Peer Reviewed |
Volume | 42 |
Issue | 1 |
Pages | 52-59 |
DOI | https://doi.org/10.1111/jvp.12719 |
Public URL | https://rvc-repository.worktribe.com/output/1384383 |
Files
11760.pdf
(2.2 Mb)
PDF
You might also like
Biased computation of Probability of Target Attainment for antimicrobial drugs
(2023)
Journal Article
Pharmacology, safety, efficacy and clinical uses of the COX‐2 inhibitor robenacoxib
(2022)
Journal Article
Levothyrox® new and old formulations: are they switchable for millions of patients?
(2019)
Journal Article
Downloadable Citations
About RVC Repository
Administrator e-mail: publicationsrepos@rvc.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search