M D'Eletto
Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75
D'Eletto, M; Rossin, F; Occhigrossi, L; Farrace, M G; Faccenda, D; Desai, R; Marchi, S; Refolo, G; Falasca, L; Antonioli, M; Ciccosanti, F; Fimia, G M; Pinton, P; Campanella, M; Piacentini, M
Authors
F Rossin
L Occhigrossi
M G Farrace
D Faccenda
R Desai
S Marchi
G Refolo
L Falasca
M Antonioli
F Ciccosanti
G M Fimia
P Pinton
M Campanella
M Piacentini
Abstract
Transglutaminase type 2 (TG2) is a multifunctional enzyme that plays a key role in mitochondria homeostasis under stressful cellular conditions. TG2 interactome analysis reveals an enzyme interaction with GRP75 (glucose-regulated protein 75). GRP75 localizes in mitochondria-associated membranes (MAMs) and acts as a bridging molecule between the two organelles by assembling the IP3R-GRP75-VDAC complex, which is involved in the transport of Ca2+ from the endoplasmic reticulum (ER) to mitochondria. We demonstrate that the TG2 and GRP75 interaction occurs in MAMs. The absence of the TG2-GRP75 interaction leads to an increase of the interaction between IP3R-3 and GRP75; a decrease of the number of ER-mitochondria contact sites; an impairment of the ER-mitochondrial Ca2+ flux; and an altered profile of the MAM proteome. These findings indicate TG2 is a key regulatory element of the MAMs.
Citation
D'Eletto, M., Rossin, F., Occhigrossi, L., Farrace, M. G., Faccenda, D., Desai, R., …Piacentini, M. (2018). Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Reports, 25(12), 3573-3581. https://doi.org/10.1016/j.celrep.2018.11.094
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 27, 2018 |
Publication Date | Dec 26, 2018 |
Deposit Date | Jan 16, 2019 |
Publicly Available Date | Nov 20, 2020 |
Journal | Cell Reports |
Print ISSN | 2211-1247 |
Publisher | Cell Press |
Peer Reviewed | Peer Reviewed |
Volume | 25 |
Issue | 12 |
Pages | 3573-3581 |
DOI | https://doi.org/10.1016/j.celrep.2018.11.094 |
Public URL | https://rvc-repository.worktribe.com/output/1384913 |
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