Oral iron exacerbates colitis and influences the intestinal microbiome

Mahalhal, A; Williams, J M; Johnson, S; Ellaby, N; Duckworth, C A; Burkitt, M D; Liu, X; Hold, G L; Campbell, B J; Pritchard, D M; Probert, C S

Abstract

Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage. The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD. Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA. Increasing or decreasing dietary iron concentration from the standard 200 ppm exacerbated both clinical and histological severity of DSS-induced colitis. DSS-treated mice provided only half the standard levels of iron ad libitum (i.e. chow containing 100 ppm iron) lost more body weight than those receiving double the amount of standard iron (i.e. 400 ppm); p

Citation

Mahalhal, A., Williams, J. M., Johnson, S., Ellaby, N., Duckworth, C. A., Burkitt, M. D., …Probert, C. S. (2018). Oral iron exacerbates colitis and influences the intestinal microbiome. PLoS ONE, 13(10), e0202460. https://doi.org/10.1371/journal.pone.0202460

• Journal Article Type: Article
• Acceptance Date: Sep 7, 2018
• Publication Date: Oct 11, 2018
• Deposit Date: Oct 19, 2018
• Publicly Available Date: Oct 23, 2018
• Journal: PLoS One
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 10
• Pages: e0202460
• DOI: https://doi.org/10.1371/journal.pone.0202460
• Public URL: https://rvc-repository.worktribe.com/output/1385658