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Metabolic perturbations associated with the consumption of a ketogenic medium-chain TAG diet in dogs with idiopathic epilepsy

Law, T; Volk, H A; Pan, Y; Zanghi, B; Want, E

Authors

T Law

H A Volk

Y Pan

B Zanghi

E Want



Abstract

Consumption of diets containing medium-chain TAG (MCT) has been shown to confer neuroprotective effects. We aim to identify the global metabolic perturbations associated with consumption of a ketogenic diet (medium-chain TAG diet (MCTD)) in dogs with idiopathic epilepsy. We used ultra-performance liquid chromatography-MS (UPLC-MS) to generate metabolic and lipidomic profiles of fasted canine serum and made comparisons between the MCTD and standardised placebo diet phases. We identified metabolites that differed significantly between diet phases using metabolite fragmentation profiles generated by tandem MS (UPLC–MS/MS). Consumption of the MCTD resulted in significant differences in serum metabolic profiles when compared with the placebo diet, where sixteen altered lipid metabolites were identified. Consumption of the MCTD resulted in reduced abundances of palmitoylcarnitine, octadecenoylcarnitine, stearoylcarnitine and significant changes, both reduced and increased abundances, of phosphatidylcholine (PC) metabolites. There was a significant increase in abundance of the saturated C17 : 0 fatty acyl moieties during the MCTD phase. Lysophosphatidylcholine (17 : 0) (P=0·01) and PC (17:0/20:4) (P=0·03) were both significantly higher in abundance during the MCTD. The data presented in this study highlight global changes in lipid metabolism, and, of particular interest, in the C17 : 0 moieties, as a result of MCT consumption. Elucidating the global metabolic response of MCT consumption will not only improve the administration of current ketogenic diets for neurological disease models but also provides new avenues for research to develop better diet therapies with improved neuroprotective efficacies. Future studies should clarify the involvement and importance of C17 : 0 moieties in endogenous MCT metabolic pathways.

Citation

Law, T., Volk, H. A., Pan, Y., Zanghi, B., & Want, E. (2018). Metabolic perturbations associated with the consumption of a ketogenic medium-chain TAG diet in dogs with idiopathic epilepsy. British Journal of Nutrition, https://doi.org/10.1017/S0007114518001617

Journal Article Type Article
Acceptance Date Jul 1, 2018
Publication Date Jul 13, 2018
Deposit Date Aug 10, 2018
Publicly Available Date Mar 28, 2024
Journal BRITISH JOURNAL OF NUTRITION
Print ISSN 0007-1145
Publisher Cambridge University Press
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1017/S0007114518001617
Public URL https://rvc-repository.worktribe.com/output/1386562

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