Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1

Lawson, J S; Syme, H M; Wheeler-Jones, C P D; Elliott, J

Abstract

Chronic kidney disease (CKD) is common in geriatric cats, and the most prevalent pathology is chronic tubulointerstitial inflammation and fibrosis. The cell type predominantly responsible for the production of extra-cellular matrix in renal fibrosis is the myofibroblast, and fibroblast to myofibroblast differentiation is probably a crucial event. The cytokine TGF-β1 is reportedly the most important regulator of myofibroblastic differentiation in other species. The aim of this study was to isolate and characterise renal fibroblasts from cadaverous kidney tissue of cats with and without CKD, and to investigate the transcriptional response to TGF-β1.

Citation

Lawson, J. S., Syme, H. M., Wheeler-Jones, C. P. D., & Elliott, J. (2018). Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1. BMC Veterinary Research, 14, 76. https://doi.org/10.1186/s12917-018-1387-2

• Journal Article Type: Article
• Acceptance Date: Feb 21, 2018
• Publication Date: Mar 9, 2018
• Deposit Date: Apr 6, 2018
• Publicly Available Date: Apr 6, 2018
• Journal: BMC Veterinary Research
• Electronic ISSN: 1746-6148
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Pages: 76
• DOI: https://doi.org/10.1186/s12917-018-1387-2
• Public URL: https://rvc-repository.worktribe.com/output/1388143