Epoxygenase inactivation exacerbates diet and aging-associated metabolic dysfunction resulting from impaired adipogenesis

Olona, A; Terra, X; Ko, J-H; Grau-Bové, C; Pinent, M; Ardevol, A; Diaz, A G; Moreno-Moral, A; Edin, M; Bishop-Bailey, D; Zeldin, D C; Aitman, T J; Petretto, E; Blay, M; Behmoaras, J

Abstract

• Cyp2j4 deletion in rats offers a simplified genetic landscape as opposed to the mice locus, which is under significant allelic expansion.

• Under different metabolic stresses (aging, cafeteria diet), Cyp2j4−/− rats show an accelerated adipogenesis, which progress towards adipocyte dysfunction.

• Adipocyte dysfunction in Cyp2j4−/− rats under cafeteria diet (CAF) is characterised by down-regulation of white adipose tissue (WAT) PPARγ and C/EBPα, adipocyte hypertrophy and extracellular matrix remodeling.

• Cyp2j4−/− rats treated with CAF display exacerbated weight gain, insulin resistance, hepatic lipid accumulation and dysregulated gluconeogenesis.

• Cyp2j4−/− rats display alternative arachidonic acid pathway usage in their adipose tissue upon CAF and aging.

Citation

Olona, A., Terra, X., Ko, J.-H., Grau-Bové, C., Pinent, M., Ardevol, A., Diaz, A. G., Moreno-Moral, A., Edin, M., Bishop-Bailey, D., Zeldin, D. C., Aitman, T. J., Petretto, E., Blay, M., & Behmoaras, J. (2018). Epoxygenase inactivation exacerbates diet and aging-associated metabolic dysfunction resulting from impaired adipogenesis. Molecular Metabolism, 11, 18-32. https://doi.org/10.1016/j.molmet.2018.03.003

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2018
• Publication Date: Mar 9, 2018
• Deposit Date: Mar 20, 2018
• Publicly Available Date: Mar 20, 2018
• Journal: Molecular Metabolism
• Electronic ISSN: 2212-8778
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Pages: 18-32
• DOI: https://doi.org/10.1016/j.molmet.2018.03.003
• Public URL: https://rvc-repository.worktribe.com/output/1388150