Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in Dogs with Chronic Enteropathies

Florey, J; Viall, A; Streu, S; DiMuro, V; Riddle, A L; Kirk, J; Perazzotti, L; Affeldt, K; Wagner, R; Vaden, S L; Harris, T; Allenspach, K

Abstract

Perinuclear antineutrophil cytoplasmic antibodies (pANCA) previously have been shown to be serum markers in dogs with chronic enteropathies, with dogs that have food‐responsive disease (FRD) having higher frequencies of seropositivity than dogs with steroid‐responsive disease (SRD). The indirect immunofluorescence (IIF) assay used in previous publications is time‐consuming to perform, with low interobserver agreement. Forty‐four dogs with FRD, 20 dogs with SRD, 20 control dogs, and 38 soft‐coated wheaten terrier (SCWT) or SCWT‐cross dogs.

Citation

Florey, J., Viall, A., Streu, S., DiMuro, V., Riddle, A. L., Kirk, J., Perazzotti, L., Affeldt, K., Wagner, R., Vaden, S. L., Harris, T., & Allenspach, K. (2017). Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in Dogs with Chronic Enteropathies. Journal of Veterinary Internal Medicine, 31(4), 1062-1066. https://doi.org/10.1111/jvim.14774

• Journal Article Type: Article
• Acceptance Date: May 6, 2017
• Publication Date: Jun 13, 2017
• Deposit Date: Sep 2, 2017
• Publicly Available Date: Sep 2, 2017
• Journal: Journal of Veterinary Internal Medicine
• Print ISSN: 0891-6640
• Electronic ISSN: 1939-1676
• Publisher: Wiley Open Access
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Issue: 4
• Pages: 1062-1066
• DOI: https://doi.org/10.1111/jvim.14774
• Public URL: https://rvc-repository.worktribe.com/output/1391527