Skip to main content

Research Repository

Advanced Search

Intestinal Preparation Techniques for Histological Analysis in the Mouse

Williams, J M; Duckworth, C A; Vowell, K; Burkitt, M D; Pritchard, D M


J M Williams

C A Duckworth

K Vowell

M D Burkitt

D M Pritchard


The murine intestinal tract represents a difficult organ system to study due to its long convoluted tubular structure, narrow diameter, and delicate mucosa which undergoes rapid changes after sampling prior to fixation. These features do not make for easy histological analysis as rapid fixation in situ, or after simple removal without careful dissection, results in poor postfixation tissue handling and limited options for high quality histological sections. Collecting meaningful quantitative data by analysis of this tissue is further complicated by the anatomical changes in structure along its length. This article describes two methods of intestinal sampling at necropsy that allow systematic histological analysis of the entire intestinal tract, either through examination of cross sections (circumferences) by the gut bundling technique or longitudinal sections by the adapted Swiss roll technique, together with basic methods for data collection.


Williams, J. M., Duckworth, C. A., Vowell, K., Burkitt, M. D., & Pritchard, D. M. (2016). Intestinal Preparation Techniques for Histological Analysis in the Mouse.

Journal Article Type Article
Acceptance Date May 1, 2016
Publication Date Jun 1, 2016
Deposit Date Jun 18, 2016
Publicly Available Date Jun 18, 2016
Journal Current Protocols in Mouse Biology
Peer Reviewed Peer Reviewed
Volume 6
Issue 2
Pages 148-68
Public URL


You might also like

Downloadable Citations