Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells

Mendes, L F; Tam, W L; Chai, Y C; Geris, L; Luyten, F P; Roberts, S J

Abstract

Successful application of cell-based strategies in cartilage and bone tissue engineering has been hampered by the lack of robust protocols to efficiently differentiate mesenchymal stem cells into the chondrogenic lineage. The development of chemically defined culture media supplemented with growth factors (GFs) has been proposed as a way to overcome this limitation. In this work, we applied a fractional design of experiment (DoE) strategy to screen the effect of multiple GFs (BMP2, BMP6, GDF5, TGF-β1, and FGF2) on chondrogenic differentiation of human periosteum-derived mesenchymal stem cells (hPDCs) in vitro. In a micromass culture (μMass) system, BMP2 had a positive effect on glycosaminoglycan deposition at day 7 (p 

Citation

Mendes, L. F., Tam, W. L., Chai, Y. C., Geris, L., Luyten, F. P., & Roberts, S. J. (2016). Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells. https://doi.org/10.1089/ten.TEC.2015.0436

• Journal Article Type: Article
• Publication Date: Apr 26, 2016
• Deposit Date: Aug 9, 2019
• Journal: Tissue Engineering Part C: Methods
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 5
• Pages: 473-86
• DOI: https://doi.org/10.1089/ten.TEC.2015.0436
• Public URL: https://rvc-repository.worktribe.com/output/1396858