B V Rose
A method for isolating and culturing placental cells from failed early equine pregnancies
Rose, B V; Cabrera-Sharp, V; Firth, M J; Barrelet, F E; Bate, S; Cameron, I J; Crabtree, J R; Crowhurst, J; McGladdery, A J; Neal, H; Pynn, J; Pynn, O D; Smith, C; Wise, Z; Verheyen, K L P; Wathes, D C; De Mestre, A M
Authors
V Cabrera-Sharp
M J Firth
F E Barrelet
S Bate
I J Cameron
J R Crabtree
J Crowhurst
A J McGladdery
H Neal
J Pynn
O D Pynn
C Smith
Z Wise
K L P Verheyen
D C Wathes
A M De Mestre
Abstract
Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.
Citation
Rose, B. V., Cabrera-Sharp, V., Firth, M. J., Barrelet, F. E., Bate, S., Cameron, I. J., …De Mestre, A. M. (2016). A method for isolating and culturing placental cells from failed early equine pregnancies. Placenta, 38, 107-111. https://doi.org/10.1016/j.placenta.2015.12.014
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 21, 2015 |
Publication Date | Jan 1, 2016 |
Deposit Date | Feb 3, 2016 |
Publicly Available Date | Nov 21, 2020 |
Journal | PLACENTA |
Print ISSN | 0143-4004 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 38 |
Pages | 107-111 |
DOI | https://doi.org/10.1016/j.placenta.2015.12.014 |
Public URL | https://rvc-repository.worktribe.com/output/1398280 |
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