A method for isolating and culturing placental cells from failed early equine pregnancies

Rose, B V; Cabrera-Sharp, V; Firth, M J; Barrelet, F E; Bate, S; Cameron, I J; Crabtree, J R; Crowhurst, J; McGladdery, A J; Neal, H; Pynn, J; Pynn, O D; Smith, C; Wise, Z; Verheyen, K L P; Wathes, D C; De Mestre, A M

Abstract

Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.

Citation

Rose, B. V., Cabrera-Sharp, V., Firth, M. J., Barrelet, F. E., Bate, S., Cameron, I. J., Crabtree, J. R., Crowhurst, J., McGladdery, A. J., Neal, H., Pynn, J., Pynn, O. D., Smith, C., Wise, Z., Verheyen, K. L. P., Wathes, D. C., & De Mestre, A. M. (2016). A method for isolating and culturing placental cells from failed early equine pregnancies. Placenta, 38, 107-111. https://doi.org/10.1016/j.placenta.2015.12.014

• Journal Article Type: Article
• Acceptance Date: Dec 21, 2015
• Publication Date: Jan 1, 2016
• Deposit Date: Feb 3, 2016
• Publicly Available Date: Feb 3, 2016
• Journal: Placenta
• Print ISSN: 0143-4004
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 38
• Pages: 107-111
• DOI: https://doi.org/10.1016/j.placenta.2015.12.014
• Public URL: https://rvc-repository.worktribe.com/output/1398280