MMP and TIMP temporal gene expression during osteocytogenesis

Prideaux, M; Staines, K A; Jones, E R; Riley, G P; Pitsillides, A A; Farquharson, C

Abstract

Osteocytes within bone differentiate from osteoblast precursors which reside in a mineralised extracellular matrix (ECM). Fully differentiated osteocytes are critical for bone development and function but the factors that regulate this differentiation process are unknown. The enzymes primarily responsible for ECM remodelling are matrix metalloproteinases (MMP); however, the expression and role of MMPs during osteocytogenesis is undefined. Here we used MLO-A5 cells to determine the temporal gene expressions of the MMP family and their endogenous inhibitors – tissue inhibitors of metalloproteinases (TIMPs) during osteocytogenesis. RT-qPCR revealed expression of 14 Mmps and 3 Timps in MLO-A5 cells. Mmp2, Mmp23 and Mmp28 were decreased concurrent with mineralisation onset (P 

Citation

Prideaux, M., Staines, K. A., Jones, E. R., Riley, G. P., Pitsillides, A. A., & Farquharson, C. (in press). MMP and TIMP temporal gene expression during osteocytogenesis. Gene Expression Patterns, 18(1-2), 29-36. https://doi.org/10.1016/j.gep.2015.04.004

• Journal Article Type: Article
• Acceptance Date: Apr 27, 2015
• Deposit Date: May 24, 2015
• Journal: Gene Expression Patterns
• Print ISSN: 1567-133X
• Publisher: Elsevier
• Volume: 18
• Issue: 1-2
• Pages: 29-36
• DOI: https://doi.org/10.1016/j.gep.2015.04.004
• Public URL: https://rvc-repository.worktribe.com/output/1401127