Skip to main content

Research Repository

Advanced Search

Effects of Ex-Vivo and In-Vivo Treatment with Probiotics on the Inflammasome in Dogs with Chronic Enteropathy

Schmitz, S; Werling, D; Allenspach, K


S Schmitz

D Werling

K Allenspach


Inflammasomes coordinate the maturation of IL-1β and IL-18 in response to danger signals. They are vital for maintenance of intestinal homeostasis and have been linked to chronic intestinal inflammation in humans. Probiotics have been advocated as treatment in intestinal inflammation. So far, no study has investigated the role of the inflammasome in canine chronic enteropathy (CE). In this study the intestinal expression of inflammasome components was assessed in CE dogs compared to controls, when treated with probiotic Enterococcus faecium (EF) ex-vivo and in-vivo. RNA extraction from endoscopic biopsies and reverse-transcriptase quantitative PCR was performed for NLRP3, casp-1, IL-1β and IL-18. Immunohistochemistry was performed to investigate protein expression in tissues. Gene expression of casp-1 and NLRP3 was lower in CE samples than controls. Ex-vivo treatment with EF reduced NLRP3 expression in control samples. Treatment of CE dogs with EF alongside dietary intervention had no effect on gene expression. In contrast, IL-1β protein expression in CE decreased with dietary treatment (but not with probiotics). The results of this study suggest that the inflammasome or its components may be partially involved in the inflammatory process seen in CE, but distinct from intestinal inflammation in humans.


Schmitz, S., Werling, D., & Allenspach, K. (in press). Effects of Ex-Vivo and In-Vivo Treatment with Probiotics on the Inflammasome in Dogs with Chronic Enteropathy. PLoS ONE, 10(3), e0120779.

Journal Article Type Article
Acceptance Date Jan 27, 2015
Deposit Date Mar 25, 2015
Publicly Available Date Mar 25, 2015
Journal PLoS One
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 10
Issue 3
Pages e0120779
Public URL


You might also like

Downloadable Citations