Urinary and faecal N-methylhistamine concentrations do not serve as markers for mast cell activation or clinical disease activity in dogs with chronic enteropathies

Anfinsen, K P; Berghoff, N; Priestnall, S L; Suchodolski, J S; Steiner, J M; Allenspach, K

Abstract

This study sought to correlate faecal and urinary N-methylhistamine (NMH) concentrations with resting versus degranulated duodenal mast cell numbers in dogs with chronic enteropathies (CE), and investigate correlations between intestinal mast cell activation and clinical severity of disease as assessed by canine chronic enteropathy clinical activity index (CCECAI), and between urinary and faecal NMH concentrations, mast cell numbers, and histopathological scores. Twenty-eight dogs with CE were included. Duodenal biopsies were stained with haematoxylin and eosin (H&E), toluidine blue, and by immunohistochemical labelling for tryptase. Duodenal biopsies were assigned a histopathological severity score, and duodenal mast cell numbers were counted in five high-power fields after metachromatic and immunohistochemical staining. Faecal and urinary NMH concentrations were measured by gas chromatography–mass spectrometry.

Citation

Anfinsen, K. P., Berghoff, N., Priestnall, S. L., Suchodolski, J. S., Steiner, J. M., & Allenspach, K. (2014). Urinary and faecal N-methylhistamine concentrations do not serve as markers for mast cell activation or clinical disease activity in dogs with chronic enteropathies. Acta Veterinaria Scandinavica, 56(90), https://doi.org/10.1186/s13028-014-0090-y

• Journal Article Type: Article
• Acceptance Date: Dec 12, 2014
• Publication Date: Dec 21, 2014
• Deposit Date: Dec 17, 2014
• Publicly Available Date: Jan 24, 2019
• Journal: Acta Veterinaria Scandinavica
• Electronic ISSN: 1751-0147
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 90
• DOI: https://doi.org/10.1186/s13028-014-0090-y
• Public URL: https://rvc-repository.worktribe.com/output/1404560