Interleukin-2 and interferon-gamma mRNA expression in canine anal furunculosis lesions and the effect of ciclosporin therapy

Tivers, M S; Catchpole, B; Gregory, S P; House, A K

Abstract

German Shepherd Dogs have an increased incidence of anal furunculosis (AF), which is a disease characterized by inflammation and ulceration of the perianal tissues. Ciclosporin, an immunosuppressive drug, has been successfully used to treat AF, suggesting that the pathogenesis of disease is likely to have an immune-mediated component. Previous research has shown that the cytokine mRNA profile in AF lesions is consistent with T cell-mediated inflammation. The aims of the current study were to quantify IL-2 and IFNγ mRNA expression in AF biopsies taken before and after treatment with ciclosporin and to compare cytokine expression with lesion severity. Twenty-two dogs with AF were recruited into the study and lesional biopsies were taken prior to ciclosporin therapy. Lesion severity was graded using a visual analogue scale. All dogs were evaluated after 4 weeks of ciclosporin therapy and, in 10 dogs with persistent disease, residual lesions were resected. RNA was extracted from AF-lesional tissue and control perianal tissue samples (n=10), which was used as the template for RT-PCR. Analysis of IL-2 and IFNγ mRNA expression was performed using real-time PCR. IL-2 and IFNγ mRNA was consistently detected in pre-treatment AF biopsies and, when quantified, this was significantly increased compared to control tissue (P

Citation

Tivers, M. S., Catchpole, B., Gregory, S. P., & House, A. K. Interleukin-2 and interferon-gamma mRNA expression in canine anal furunculosis lesions and the effect of ciclosporin therapy. Veterinary Immunology and Immunopathology, 125(1/2), 31-36. https://doi.org/10.1016/j.vetimm.2008.04.018

• Journal Article Type: Article
• Deposit Date: Nov 12, 2014
• Journal: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
• Print ISSN: 0165-2427
• Publisher: Elsevier
• Volume: 125
• Issue: 1/2
• Pages: 31-36
• DOI: https://doi.org/10.1016/j.vetimm.2008.04.018
• Public URL: https://rvc-repository.worktribe.com/output/1431263