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Treatment With Hydrolyzed Diet Supplemented With Prebiotics and Glycosaminoglycans Alters Lipid Metabolism in Canine Inflammatory Bowel Disease

Lacombe, Véronique Anne; Capela, F; Silva; Allenspach, Karin; Ambrosini, Yoko M; Neuber, Sebastian; Borcherding, Dana; Seo, Yeon-Jung; Segarra, Sergi; Glanemann, Barbara; Garden, Oliver A; Müller, Udo; Gordian Adam, M; Dang, Viet; Borts, David; Atherly, Todd; Willette, Auriel A; Jergens, Albert; Mochel, Jonathan P; Allenspach, Karin

Authors

Véronique Anne Lacombe

F Capela

Silva

Karin Allenspach

Yoko M Ambrosini

Sebastian Neuber

Dana Borcherding

Yeon-Jung Seo

Sergi Segarra

Barbara Glanemann

Oliver A Garden

Udo Müller

M Gordian Adam

Viet Dang

David Borts

Todd Atherly

Auriel A Willette

Albert Jergens

Jonathan P Mochel

Karin Allenspach



Abstract

Canine inflammatory bowel disease (IBD) is a chronic, immunologically mediated intestinal disorder, resulting from the complex interaction of genetic, environmental and immune factors. Hydrolyzed diets are used in dogs with food-responsive diarrhea (FRD) to reduce adverse responses to immunostimulatory proteins. Prebiotics (PRBs) and glycosaminoglycans (GAGs) have previously been demonstrated to show anti-inflammatory activity in the intestinal mucosa. Notably, hydrolyzed diets combined with the administration of PRBs and GAGs offer a promising approach for the treatment of canine IBD. Our aim was to investigate the effects of hydrolyzed diet and GAG+PRB co-treatment on the serum metabolomic profile of IBD dogs. Dogs with IBD randomly received either hydrolyzed diet supplemented with GAGs and PRBs (treatment 1) or hydrolyzed diet alone (treatment 2) for 10 weeks. A targeted metabolomics approach using mass spectrometry was performed to quantify changes in the serum metabolome before and after treatment and between treatment 1 and 2. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), hierarchical cluster analysis (HCA) and univariate statistics were used to identify differences between the treatment groups. PCA, PLS-DA, and HCA showed a clear clustering of IBD dogs before and after hydrolyzed diet, indicating that the treatment impacted the serum metabolome. Univariate analysis revealed that most of the altered metabolites were involved in lipid metabolism. The most impacted lipid classes were components of cell membranes, including glycerophospholipids, sphingolipids, and di-and triglycerides. In addition, changes in serum metabolites after GAG+PRB co-treatment suggested a possible additional beneficial effect on the lipid metabolism in IBD dogs. In conclusion, the present Ambrosini et al. Lipid Metabolism in Canine IBD study showed a significant increase in metabolites that protect gut cell membrane integrity in response to hydrolyzed diet alone or in combination with GAG+PRB co-treatment. Administration of such treatment over 70 days improved selected serum biomarkers of canine IBD, possibly indicating improved intestinal membrane integrity.

Citation

Lacombe, V. A., Capela, F., Silva, Allenspach, K., Ambrosini, Y. M., Neuber, S., …Allenspach, K. (2020). Treatment With Hydrolyzed Diet Supplemented With Prebiotics and Glycosaminoglycans Alters Lipid Metabolism in Canine Inflammatory Bowel Disease. Frontiers in Veterinary Science, 7, -. https://doi.org/10.3389/fvets.2020.00451

Journal Article Type Article
Acceptance Date Jun 22, 2020
Online Publication Date Jul 30, 2020
Publication Date 2020-07
Deposit Date Jul 7, 2022
Publicly Available Date Jul 13, 2022
Journal Frontiers in Veterinary Science | www.frontiersin.org
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 7
Pages -
DOI https://doi.org/10.3389/fvets.2020.00451
Keywords metabolomics (OMICS); inflammatory bowel disease; dogs; serum; partial least-squares- discriminant analysis

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