C Arthurs
Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2
Arthurs, C; Suarez-Bonnet, A; Willis, C; Xie, B; Machulla, N; Mair, T S; Cao, K; Millar, M; Thrasivoulou, C; Priestnall, S L; Ahmed, A
Authors
A Suarez-Bonnet
C Willis
B Xie
N Machulla
T S Mair
K Cao
M Millar
C Thrasivoulou
S L Priestnall
A Ahmed
Abstract
Equine penile squamous cell carcinoma (EpSCC) is a relatively common cutaneous neoplasm with a poor prognosis. In this study, we aimed to determine the protein expression and colocalisation of FRA1, c-Myc, Cyclin D1, and MMP7 in normal (NT), tumour (T), hyperplastic epidermis and/or squamous papilloma (Hyp/Pap), poorly-differentiated (PDSCC), or well-differentiated (WDSCC) EpSCC using a tissue array approach. Further objectives were to correlate protein expression to (i) levels of inflammation, using a convolutional neural network (ii) equine papillomavirus 2 (EcPV2) infection, detected using PCR amplification. We found an increase in expression of FRA1 in EpSCC compared to NT samples. c-Myc expression was higher in Hyp/Pap and WDSCC but not PDSCC whereas MMP7 was reduced in WDSCC compared with NT. There was a significant increase in the global intersection coefficient (GIC) of FRA1 with MMP7, c-Myc, and Cyclin D1 in EpSCC. Conversely, GIC for MMP7 with c-Myc was reduced in EpSCC tissue. Inflammation was positively associated with EcPV2 infection in both NT and EpSCC but not Hyp/Pap. Changes in protein expression could be correlated with EcPV2 for Cyclin D1 and c-Myc. Our results evaluate novel biomarkers of EpSCC and a putative correlation between the expression of biomarkers, EcPV2 infection and inflammation.
Citation
Arthurs, C., Suarez-Bonnet, A., Willis, C., Xie, B., Machulla, N., Mair, T. S., …Ahmed, A. (2020). Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2. Scientific Reports, 10(1), https://doi.org/10.1038/s41598-020-64014-3
Journal Article Type | Article |
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Acceptance Date | Mar 27, 2020 |
Publication Date | May 12, 2020 |
Deposit Date | May 27, 2020 |
Publicly Available Date | Nov 20, 2020 |
Journal | Scientific Reports |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 1 |
DOI | https://doi.org/10.1038/s41598-020-64014-3 |
Public URL | https://rvc-repository.worktribe.com/output/1377320 |
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