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Toward Improving Interventions Against Toxoplasmosis by Identifying Routes of Transmission Using Sporozoite- specific Serological Tools

Walker, M; Webster, J P; Milne, Gregory


M Walker

J P Webster

Gregory Milne


Background. Horizontal transmission of Toxoplasma gondii occurs primarily via ingestion of environmental oocysts or con- sumption of undercooked/raw meat containing cyst-stage bradyzoites. The relative importance of these 2 transmission routes remains unclear. Oocyst infection can be distinguished from bradyzoite infection by identification of immunoglobulin G (IgG) antibodies against T. gondii embryogenesis-related protein (TgERP). These antibodies are, however, thought to persist for only 6–8 months in human sera, limiting the use of TgERP serology to only those patients recently exposed to T. gondii. Yet recent sero- logical survey data indicate a more sustained persistence of anti-TgERP antibodies. Elucidating the duration of anti-TgERP IgG will help to determine whether TgERP serology has epidemiological utility for quantifying the relative importance of different routes of T. gondii transmission.
Methods. We developed a serocatalytic mathematical model to capture the change in seroprevalence of non-stage-specific IgG and anti-TgERP IgG antibodies with human age. The model was fitted to published datasets collected in an endemic region of Brazil to estimate the duration of anti-TgERP IgG antibodies, accounting for variable age–force of infection profiles and uncertainty in the diagnostic performance of TgERP serology.
Results. We found that anti-TgERP IgG persists for substantially longer than previously recognized, with estimates ranging from 8.3 to 41.1 years. The Brazilian datasets were consistent with oocysts being the predominant transmission route in these settings.
Conclusions. The longer than previously recognized duration of anti-TgERP antibodies indicates that anti-TgERP serology could be a useful tool for delineating T. gondii transmission routes in human populations. TgERP serology may therefore be an important epidemiological tool for informing the design of tailored, setting-specific public health information campaigns and interventions.


Walker, M., Webster, J. P., & Milne, G. (2020). Toward Improving Interventions Against Toxoplasmosis by Identifying Routes of Transmission Using Sporozoite- specific Serological Tools. Clinical Infectious Diseases,

Journal Article Type Article
Acceptance Date Apr 2, 2020
Publication Date Apr 13, 2020
Deposit Date Apr 27, 2020
Publicly Available Date Apr 27, 2020
Journal Clinical Infectious Diseases
Print ISSN 1058-4838
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Public URL


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