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Polyhexamethylene Biguanide: Polyurethane Blend Nanofibrous Membranes for Wound Infection Control

Worsley, A; Vassileva, K; Tsui, J; Song, W; Good, L


A Worsley

K Vassileva

J Tsui

W Song

L Good


Polyhexamethylene biguanide (PHMB) is a broad-spectrum antiseptic which avoids many efficacy and toxicity problems associated with antimicrobials, in particular, it has a low risk of loss of susceptibility due to acquired antimicrobial resistance. Despite such advantages, PHMB is not widely used in wound care, suggesting more research is required to take full advantage of PHMB’s properties. We hypothesised that a nanofibre morphology would provide a gradual release of PHMB, prolonging the antimicrobial effects within the therapeutic window. PHMB:polyurethane (PU) electrospun nanofibre membranes were prepared with increasing PHMB concentrations, and the effects on antimicrobial activities, mechanical properties and host cell toxicity were compared. Overall, PHMB:PU membranes displayed a burst release of PHMB during the first hour following PBS immersion (50.5–95.9% of total released), followed by a gradual release over 120 h (≤25 wt % PHMB). The membranes were hydrophilic (83.7–53.3°), gradually gaining hydrophobicity as PHMB was released. They displayed superior antimicrobial activity, which extended past the initial release period, retained PU hyperelasticity regardless of PHMB concentration (collective tensile modulus of 5–35% PHMB:PU membranes, 3.56 ± 0.97 MPa; ultimate strain, >200%) and displayed minimal human cell toxicity (


Worsley, A., Vassileva, K., Tsui, J., Song, W., & Good, L. (2019). Polyhexamethylene Biguanide: Polyurethane Blend Nanofibrous Membranes for Wound Infection Control. Polymers, 11(5), 915.

Journal Article Type Article
Acceptance Date May 20, 2019
Publication Date May 22, 2019
Deposit Date May 30, 2019
Publicly Available Date May 30, 2019
Journal Polymers
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 11
Issue 5
Pages 915
Public URL