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Palmitoleic acid prevents palmitic acid-induced macrophage activation and consequent p38 MAPK-mediated-skeletal muscle insulin resistance

Talbot, N A; Wheeler-Jones, C P D; Cleasby, M E

Authors

N A Talbot

C P D Wheeler-Jones

M E Cleasby



Abstract

Obesity and saturated fatty acid (SFA) treatment are both associated with skeletal muscle insulin resistance (IR) and increased macrophage infiltration. However, the relative effects of SFA and unsaturated fatty acid (UFA)-activated macrophages on muscle are unknown. Here, macrophages were treated with palmitic acid, palmitoleic acid or both and the effects of the conditioned medium (CM) on C2C12 myotubes investigated. CM from palmitic acid-treated J774s (palm-mac-CM) impaired insulin signalling and insulin-stimulated glycogen synthesis, reduced Inhibitor κBα and increased phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase in myotubes. p38 MAPK inhibition or siRNA partially ameliorated these defects, as did addition of tumour necrosis factor-α blocking antibody to the CM. Macrophages incubated with both FAs generated CM that did not induce IR, while palmitoleic acid-mac-CM alone was insulin sensitising. Thus UFAs may improve muscle insulin sensitivity and counteract SFA-mediated IR through an effect on macrophage activation.

Citation

Talbot, N. A., Wheeler-Jones, C. P. D., & Cleasby, M. E. (2014). Palmitoleic acid prevents palmitic acid-induced macrophage activation and consequent p38 MAPK-mediated-skeletal muscle insulin resistance. Molecular and Cellular Endocrinology, 393(1-2), 129-142. https://doi.org/10.1016/j.mce.2014.06.010

Journal Article Type Article
Acceptance Date Jun 13, 2014
Publication Date Aug 1, 2014
Deposit Date Nov 11, 2014
Publicly Available Date Nov 21, 2020
Journal MOLECULAR AND CELLULAR ENDOCRINOLOGY
Print ISSN 0303-7207
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 393
Issue 1-2
Pages 129-142
DOI https://doi.org/10.1016/j.mce.2014.06.010
Public URL https://rvc-repository.worktribe.com/output/1405152

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