Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of salmonella typhimurium in the chicken

Parsons, B N; Wigley, P; Simpson, H; Williams, J M; Humphrey, S; Salisbury, A-M; Watson, A J M; Fry, S C; O'Brien, D; Roberts, C L; O'Kennedy, N; Keita, A V; Soderholm, J D; Rhodes, J M; Campbell, B J

Abstract

Soluble fibres (non-starch polysaccharides, NSP) from edible plants but particularly plantain banana (Musa spp.), have been shown in vitro and ex vivo to prevent various enteric pathogens from adhering to, or translocating across, the human intestinal epithelium, a property that we have termed contrabiotic. Here we report that dietary plantain fibre prevents invasion of the chicken intestinal mucosa by Salmonella. In vivo experiments were performed with chicks fed from hatch on a pellet diet containing soluble plantain NSP (0 to 200 mg/d) and orally infected with S.Typhimurium 4/74 at 8 d of age. Birds were sacrificed 3, 6 and 10 d post-infection. Bacteria were enumerated from liver, spleen and caecal contents. In vitro studies were performed using chicken caecal crypts and porcine intestinal epithelial cells infected with Salmonella enterica serovars following pre-treatment separately with soluble plantain NSP and acidic or neutral polysaccharide fractions of plantain NSP, each compared with saline vehicle. Bacterial adherence and invasion were assessed by gentamicin protection assay. In vivo dietary supplementation with plantain NSP 50 mg/d reduced invasion by S.Typhimurium, as reflected by viable bacterial counts from splenic tissue, by 98.9% (95% CI, 98.1–99.7; P

Citation

Parsons, B. N., Wigley, P., Simpson, H., Williams, J. M., Humphrey, S., Salisbury, A.-M., Watson, A. J. M., Fry, S. C., O'Brien, D., Roberts, C. L., O'Kennedy, N., Keita, A. V., Soderholm, J. D., Rhodes, J. M., & Campbell, B. J. (2014). Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of salmonella typhimurium in the chicken. PLoS ONE, https://doi.org/10.1371/journal.pone.0087658

• Journal Article Type: Article
• Acceptance Date: Dec 28, 2013
• Publication Date: Feb 3, 2014
• Deposit Date: Jul 20, 2018
• Publicly Available Date: Jul 25, 2018
• Journal: PLoS One
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1371/journal.pone.0087658
• Public URL: https://rvc-repository.worktribe.com/output/1406029