Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

Wass, M N; Stanway, R; Blagborough, A M; Lal, K; Prieto, J H; Raine, D; Sternberg, M J E; Talman, A M; Tomley, F M; Yates, J; Sinden, R E

doi:10.1017/s0031182012000133

Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

Wass, M N; Stanway, R; Blagborough, A M; Lal, K; Prieto, J H; Raine, D; Sternberg, M J E; Talman, A M; Tomley, F M; Yates, J; Sinden, R E

Authors

M N Wass

R Stanway

A M Blagborough

K Lal

J H Prieto

D Raine

M J E Sternberg

A M Talman

F M Tomley

J Yates

R E Sinden

Abstract

Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.

Citation

Wass, M. N., Stanway, R., Blagborough, A. M., Lal, K., Prieto, J. H., Raine, D., Sternberg, M. J. E., Talman, A. M., Tomley, F. M., Yates, J., & Sinden, R. E. (2012). Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls. Parasitology, 139(9), 1131-1145. https://doi.org/10.1017/s0031182012000133

Journal Article Type	Article
Acceptance Date	Jan 11, 2012
Publication Date	Feb 16, 2012
Deposit Date	Nov 11, 2014
Publicly Available Date	Jan 24, 2019
Journal	Parasitology
Print ISSN	0031-1820
Electronic ISSN	1469-8161
Publisher	Cambridge University Press
Peer Reviewed	Peer Reviewed
Volume	139
Issue	9
Pages	1131-1145
DOI	https://doi.org/10.1017/s0031182012000133
Public URL	https://rvc-repository.worktribe.com/output/1413317
Additional Information	Corporate Creators : Bern, Switzerland, Imperial College London, Scripps Research Inst, CA, USA, Yale