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Bone marrow lesions: plugging the holes in our knowledge using animal models (2023)
Journal Article
Hansen, R., Chenu, C., Sofat, N., & Pitsillides, A. (2023). Bone marrow lesions: plugging the holes in our knowledge using animal models. Nature Reviews Rheumatology, https://doi.org/10.1038/s41584-023-00971-z

Bone marrow lesions (BMLs), which are early signs of osteoarthritis (OA) that are associated with the presence, onset and severity of pain, represent an emerging imaging biomarker and clinical target. Little is known, however, regarding their early s... Read More about Bone marrow lesions: plugging the holes in our knowledge using animal models.

Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice (2023)
Journal Article
Monzem, S., Valkani, D., Evans, L., Chang, Y., & Pitsillides, A. (2023). Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice. BONE, 170, https://doi.org/10.1016/j.bone.2023.116720

Beneficial effects of intermittent parathyroid hormone (PTH) on bone mass and architecture are described to either simply add to, or to synergise with those of mechanical loading. We evaluate whether interaction with in vivo loading is reinforced by... Read More about Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice.

Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting (2022)
Journal Article
Santos-Beato, P., Midha, S., Pitsillides, A., Miller, A., Torii, R., & Kalaskar, D. (2022). Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting. Journal of Tissue Engineering, 13, https://doi.org/10.1177/20417314221133480

Multiple prevalent diseases, such as osteoarthritis (OA), for which there is no cure or full understanding, affect the osteochondral unit; a complex interface tissue whose architecture, mechanical nature and physiological characteristics are still ye... Read More about Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting.

Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy (2022)
Journal Article
Hsu, S., Stephen, L., Dillon, S., Milne, E., Javaheri, B., Pitsillides, A., …Farquharson, C. (2022). Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy. Journal of Endocrinology, 254(3), 167-181. https://doi.org/10.1530/JOE-22-0097

Patients with advanced chronic kidney disease (CKD) often present with skeletal abnormalities, a condition known as renal osteodystrophy (ROD). While tissue non-specific alkaline phosphatase (TNAP) and PHOSPHO1 are critical for bone mineralization, t... Read More about Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy.

Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis (2022)
Journal Article
Jones, G., Pitsillidies, A., & Meeson, R. (2022). Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2022.789898

Osteoarthritis (OA) is the most common orthopaedic condition in dogs, characterised as chronic, painful end-point of a synovial joint with limited therapeutic options other than palliative pain control or surgical salvage. Since the 1970s, radiograph... Read More about Moving beyond the limits of detection: the past, the present, and the future of diagnostic imaging in canine osteoarthritis.

An examination of two different approaches for the study of femoral neck fracture: towards a more relevant rodent model (2021)
Journal Article
Monzem, S., Gohin, S., Yagüe Ballester, R., Lopes de Souza, R., Meeson, R., & Anthony Pitsillides, A. (2021). An examination of two different approaches for the study of femoral neck fracture: towards a more relevant rodent model. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, https://doi.org/10.1177/09544119211053057

Femoral neck fractures are a massive personal and health programme burden. Methods to study femoral neck strength, across its combined trabecular and cortical components are therefore essential. Rodent ovariectomy-induced osteoporosis models are comm... Read More about An examination of two different approaches for the study of femoral neck fracture: towards a more relevant rodent model.

CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4 (2021)
Journal Article
Marr, N., Meeson, R., Kelly, E., Fang, Y., Peffers, M., Pitsillides, A., …Thorpe, C. (2021). CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4. International Journal of Molecular Sciences, https://doi.org/10.3390/ijms22189729

The interfascicular matrix (IFM) binds tendon fascicles and contains a population of morphologically distinct cells; however the role of IFM-localised cell populations in tendon repair remains to be determined. The basement membrane protein laminin-α... Read More about CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4.

Sciatic neurectomy-related cortical bone loss exhibits delayed onset yet stabilises more rapidly than trabecular bone (2021)
Journal Article
Monzem, S., Javaheri, B., de Souza, R. L., & Pitsillides, A. A. (2021). Sciatic neurectomy-related cortical bone loss exhibits delayed onset yet stabilises more rapidly than trabecular bone. Bone Reports, 101116. https://doi.org/10.1016/j.bonr.2021.101116

Disuse osteoporosis occurs after extended periods of bed rest or nerve damage leading to increased risk of fracture. It remains to be established, however, whether the trajectory of bone loss is equivalent in bone's cortical and trabecular compartmen... Read More about Sciatic neurectomy-related cortical bone loss exhibits delayed onset yet stabilises more rapidly than trabecular bone.

A new straightforward method for semi-automated segmentation of trabecular bone from cortical bone in diverse and challenging morphologies (2021)
Journal Article
Pitsillides, A., Herbst, E., Felder, A., Evans, L., Anjami, S., & Javaheri, B. (2021). A new straightforward method for semi-automated segmentation of trabecular bone from cortical bone in diverse and challenging morphologies. Royal Society Open Science, https://doi.org/10.1098/rsos.210408

Many physiological, biomechanical, evolutionary and clinical studies that explore skeletal structure and function require successful separation of trabecular from cortical compartments of a bone that has been imaged by X-ray micro-computed tomography... Read More about A new straightforward method for semi-automated segmentation of trabecular bone from cortical bone in diverse and challenging morphologies.

Systematic analysis reveals that colony housing aligns gait profiles and strengthens link between 1 histological and micro-CT bone markers in rat models of osteoarthritis 2 Short running title: Colony housing strengthens osteoarthritis research (2021)
Journal Article
Chang, Y., Menges, S., Westhof, A., Kleinschmidt-Doerr, K., Brenneis, C., Pitsillides, A. A., & Chang, Y. (in press). Systematic analysis reveals that colony housing aligns gait profiles and strengthens link between 1 histological and micro-CT bone markers in rat models of osteoarthritis 2 Short running title: Colony housing strengthens osteoarthritis research. FASEB Journal, -. https://doi.org/10.1096/fj.202002009R

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon (2020)
Journal Article
Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., …Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individu... Read More about Heterogeneity of proteome dynamics between connective tissue phases of adult tendon.

Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity (2020)
Journal Article
Gohin, S., Javaheri, B., Hopkinson, M., Pitsillides, A. A., Arnett, T. R., & Chenu, C. (2020). Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity. Journal of Applied Physiology,

Blood supply is essential for osteogenesis, yet its relationship to load-related increases in bone mass is poorly defined. Herein, we aim to investigate the link between load-induced osteogenesis and the blood supply (bone perfusion and vascular poro... Read More about Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity.

Lasting organ-level bone mechanoadaptation is unrelated to local strain (2020)
Journal Article
Javaheri, B., Razi, H., Gohin, S., Wylie, S., Chang, Y. M., Salmon, P., …Pitsillides, A. A. (2020). Lasting organ-level bone mechanoadaptation is unrelated to local strain. Science Advances, 6(10), https://doi.org/10.1126/sciadv.aax8301

Bones adapt to mechanical forces according to strict principles predicting straight shape. Most bones are, however, paradoxically curved. To solve this paradox, we used computed tomography–based, four-dimensional imaging methods and computational ana... Read More about Lasting organ-level bone mechanoadaptation is unrelated to local strain.

Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity (2020)
Journal Article
Staines, K., Hopkinson, M., Dillon, S., Stephen, L., Fleming, R., Sophocleous, A., …Farquharson, C. (2020). Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity. Bioscience Reports, 40, https://doi.org/10.1042/BSR20190329

E11/Podoplanin (Pdpn) is implicated in early osteocytogenesis and the formation of osteocyte dendrites. This dendritic network is critical for bone modelling/remodelling, through the production of receptor activator of nuclear factor κ B (RANK)-ligan... Read More about Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity.

Aging and Mechanoadaptive Responsiveness of Bone (2019)
Journal Article
Javaheri, B., & Pitsillides, A. A. (2019). Aging and Mechanoadaptive Responsiveness of Bone. Current Osteoporosis Reports, 17(6), 560-569. https://doi.org/10.1007/s11914-019-00553-7

Osteoporosis is an age-related disorder characterized by bone loss and increased fracture susceptibility. Whether this is due to reduced loading in less active elderly individuals or inherent modifications in bone cells is uncertain. We suppose that... Read More about Aging and Mechanoadaptive Responsiveness of Bone.