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All Outputs (19)

Identifying Fibroblast Growth Factor Receptor 3 as a Mediator of Periosteal Osteochondral Differentiation through the Construction of microRNA-Based Interaction Networks (2023)
Journal Article
Wells, L. M., Roberts, H. C., Luyten, F. P., & Roberts, S. J. (2023). Identifying Fibroblast Growth Factor Receptor 3 as a Mediator of Periosteal Osteochondral Differentiation through the Construction of microRNA-Based Interaction Networks. Biology, 12(11), 1381. https://doi.org/10.3390/biology12111381

Human periosteum-derived progenitor cells (hPDCs) have the ability to differentiate towards both the chondrogenic and osteogenic lineages. This coordinated and complex osteochondrogenic differentiation process permits endochondral ossification and is... Read More about Identifying Fibroblast Growth Factor Receptor 3 as a Mediator of Periosteal Osteochondral Differentiation through the Construction of microRNA-Based Interaction Networks.

Novel insights on the effect of sclerostin on bone and other organs (2023)
Journal Article
Dreyer, T., Keen, J., Wells, L., & Roberts, S. (2023). Novel insights on the effect of sclerostin on bone and other organs. Journal of Endocrinology, 257(2), https://doi.org/10.1530/JOE-22-0209

As a key regulator of bone homeostasis, sclerostin has garnered a lot of interest over the last two decades. Although sclerostin is primarily expressed by osteocytes and is well known for its role in bone formation and remodelling, it is also express... Read More about Novel insights on the effect of sclerostin on bone and other organs.

The Prrx1eGFP Mouse Labels the Periosteum During Development and a Subpopulation of Osteogenic Periosteal Cells in the Adult (2022)
Journal Article
Brown, S., Malik, S., Aljammal, M., O'Flynn, A., Hobbs, C., Shah, M., …Logan, M. P. (in press). The Prrx1eGFP Mouse Labels the Periosteum During Development and a Subpopulation of Osteogenic Periosteal Cells in the Adult. JBMR Plus, e10707, https://doi.org/10.1002/jbm4.10707

The identity of the cells that form the periosteum during development is controversial with current dogma suggesting these are derived from a Sox9-positive progenitor. Herein, we characterize a newly created Prrx1eGFP reporter transgenic mouse line d... Read More about The Prrx1eGFP Mouse Labels the Periosteum During Development and a Subpopulation of Osteogenic Periosteal Cells in the Adult.

Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells (2022)
Journal Article
Vas, W., Shah, M., Roberts, H., & Roberts, S. (2022). Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells. Bioengineering, 9(5), https://doi.org/10.3390/bioengineering9050203

The control of cell behaviour in an effort to create highly homogeneous cultures is becoming an area of intense research, both to elucidate fundamental biology and for regenerative applications. The extracellular matrix (ECM) controls many cellular p... Read More about Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells.

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension (2022)
Journal Article
Al Hosni, R., Bozec, L., Roberts, S. J., & Cheema, U. (2022). Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension. iScience, 25(4), 104059. https://doi.org/10.1016/j.isci.2022.104059

The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenc... Read More about Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications. (2022)
Journal Article
Guest, D. J., Dudhia, J., Smith, R. K., Roberts, S., Conzemius, M., Innes, J. F., …Meeson, R. L. (2022). Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2022.817041

Mesenchymal stromal/stem cells (MSCs) are being used in an increasingly broad range of clinical applications in horses, dogs and cats. In this position statement, we aim to provide guidelines to improve reporting research involving the use of MSCs in... Read More about Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications..

Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects (2021)
Journal Article
Tam, W. L., Freitas Mendes, L., Chen, X., Lesage, R., Van Hoven, I., Leysen, E., …Luyten, F. P. (2021). Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects. Stem Cell Research and Therapy, 12(1), https://doi.org/10.1186/s13287-021-02580-7

Background Bones have a remarkable capacity to heal upon fracture. Yet, in large defects or compromised conditions healing processes become impaired, resulting in delayed or non-union. Current therapeutic approaches often utilize autologous or allog... Read More about Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects.

Dual neutralisation of IL-17F and IL-17A with bimekizumab blocks inflammation-driven osteogenic differentiation of human periosteal cells (2020)
Journal Article
Shah, M., Maroof, A., Gikas, P., Mittal, G., Keen, R., Baeten, D., …Roberts, S. J. (2020). Dual neutralisation of IL-17F and IL-17A with bimekizumab blocks inflammation-driven osteogenic differentiation of human periosteal cells. RMD Open, 6(2), e001306. https://doi.org/10.1136/rmdopen-2020-001306

Objectives Interleukin (IL)-17 signalling has been shown to be a key regulator of disease in ankylosing spondylitis (AS) with several IL-17 blockers currently clinically approved. Despite this, the role of IL-17 in bone pathology is poorly understo... Read More about Dual neutralisation of IL-17F and IL-17A with bimekizumab blocks inflammation-driven osteogenic differentiation of human periosteal cells.

Mapping human serum–induced gene networks as a basis for the creation of biomimetic periosteum for bone repair (2020)
Journal Article
Al Hosni, R., Shah, M., Cheema, U., Roberts, H., Luyten, F., & Roberts, S. (2020). Mapping human serum–induced gene networks as a basis for the creation of biomimetic periosteum for bone repair. Cytotherapy,

The periosteum is a highly vascularised, collagen-rich tissue that plays a crucial role in directing bone repair. This is orchestrated primarily by its resident progenitor cell population. Indeed, preservation of periosteum integrity is critical for... Read More about Mapping human serum–induced gene networks as a basis for the creation of biomimetic periosteum for bone repair.

Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo. (2018)
Journal Article
Mendes, L. F., Katairi, H., Tam, W. L., Chai, Y. C., Geris, L., Roberts, S. J., & Luyten, F. P. (2018). Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo. https://doi.org/10.1186/s13287-018-0787-3

Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model (2018)
Journal Article
Sahakyan, V., Duelen, R., Tam, W. L., Roberts, S. J., Grosemans, H., Berckmans, P., …Sampaolesi, M. (2018). Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model. Nature, 8, 2942. https://doi.org/10.1038/s41598-018-21103-8

Neural tube defects (NTDs) are severe congenital abnormalities, caused by failed closure of neural tube during early embryonic development. Periconceptional folic acid (FA) supplementation greatly reduces the risk of NTDs. However, the molecular mech... Read More about Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model.

Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells (2016)
Journal Article
Mendes, L. F., Tam, W. L., Chai, Y. C., Geris, L., Luyten, F. P., & Roberts, S. J. (2016). Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells. https://doi.org/10.1089/ten.TEC.2015.0436

Successful application of cell-based strategies in cartilage and bone tissue engineering has been hampered by the lack of robust protocols to efficiently differentiate mesenchymal stem cells into the chondrogenic lineage. The development of chemicall... Read More about Combinatorial Analysis of Growth Factors Reveals the Contribution of Bone Morphogenetic Proteins to Chondrogenic Differentiation of Human Periosteal Cells.

Osteomimetic matrix components alter cell migration and drug response in a 3D tumour-engineered osteosarcoma model
Journal Article
Pavlou, M., Shah, M., Gikas, P., Briggs, T., Roberts, S. J., & Cheema, U. (in press). Osteomimetic matrix components alter cell migration and drug response in a 3D tumour-engineered osteosarcoma model. Acta Biomaterialia, 96, 247-257. https://doi.org/10.1016/j.actbio.2019.07.011

vulnerable to recurring disease and pulmonary metastases. Developing 3D in vitro disease models to serve as a test bed for personalised treatment is a promising approach to address this issue. This study describes the generation of 3D osteosarcoma mo... Read More about Osteomimetic matrix components alter cell migration and drug response in a 3D tumour-engineered osteosarcoma model.

Early BMP, Wnt and Ca(2+)/PKC pathway activation predicts the bone forming capacity of periosteal cells in combination with calcium phosphates
Journal Article
Bolander, J., Chai, Y. C., Geris, L., Schrooten, J., Lambrechts, D., Roberts, S. J., & Luyten, F. P. (in press). Early BMP, Wnt and Ca(2+)/PKC pathway activation predicts the bone forming capacity of periosteal cells in combination with calcium phosphates. Biomaterials, 106-18. https://doi.org/10.1016/j.biomaterials.2016.01.059

The development of osteoinductive calcium phosphate- (CaP) based biomaterials has, and continues to be, a major focus in the field of bone tissue engineering. However, limited insight into the spatiotemporal activation of signalling pathways has hamp... Read More about Early BMP, Wnt and Ca(2+)/PKC pathway activation predicts the bone forming capacity of periosteal cells in combination with calcium phosphates.

Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro and trigger endochondral ossification in vivo
Journal Article
Tam, W. L., Df, O., Hiramatsu, K., Tsumaki, N., Luyten, F. P., & Roberts, S. J. (in press). Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro and trigger endochondral ossification in vivo. Cellular Reprogramming, 16(1), 29-39. https://doi.org/10.1089/cell.2013.0060

Strategies for bone regeneration are undergoing a paradigm shift, moving away from the replication of end-stage bone tissue and instead aiming to recapture the initial events of fracture repair. Although this is known to resemble endochondral bone fo... Read More about Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro and trigger endochondral ossification in vivo.