Phylogenetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini sensu lato from Sakon Nakhon, Thailand by mitochondrial and nuclear DNA sequencing

Pitaksakulrat, O; Webster, B L; Webster, J P; Laha, T; Saijuntha, W; Lamberton, P H L; Kiatsopit, N; Andrews, R H; Petney, T N; Sithithaworn, P

Abstract

The liver fluke Opisthorchis viverrini sensu lato causes serious public-health problems in Northeast Thailand and Southeast Asian countries. A hypothesis has been proposed that O. viverrini represents a species complex with varying levels of genetic differentiation in Thailand and Lao PDR. This study aimed to clarify whether O. viverrini populations can be genetically divided into separate taxa. We collected O. viverrini s.l. from eight different locations in Lao PDR and Thailand. The results of nad1, cox1, CF-int6, Pm-int9, ITS2 and 28S rDNA sequence analysis revealed that sub-structuring occurred between the eight populations. We found that O. viverrini s.l. from Sakon Nakhon (SK), Thailand, shows significant genetic differentiation (P 

Citation

Pitaksakulrat, O., Webster, B. L., Webster, J. P., Laha, T., Saijuntha, W., Lamberton, P. H. L., Kiatsopit, N., Andrews, R. H., Petney, T. N., & Sithithaworn, P. (2018). Phylogenetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini sensu lato from Sakon Nakhon, Thailand by mitochondrial and nuclear DNA sequencing. https://doi.org/10.1016/j.meegid.2018.04.022

• Journal Article Type: Article
• Acceptance Date: Apr 17, 2018
• Publication Date: Apr 18, 2018
• Deposit Date: Apr 26, 2018
• Publicly Available Date: May 1, 2019
• Journal: Infections, Genetics and Evolution
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1016/j.meegid.2018.04.022
• Public URL: https://rvc-repository.worktribe.com/output/1387582